King Abdullah Institute for Nanotechnology, King Saud University, Riyadh 11451, Saudi Arabia.
Department of Biochemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
Molecules. 2020 Mar 3;25(5):1137. doi: 10.3390/molecules25051137.
This study aimed to generate a comparative data on biological response of yttrium oxide nanoparticles (YO NPs) with the antioxidant CeO NPs and pro-oxidant ZnO NPs. Sizes of YO NPs were found to be in the range of 35±10 nm as measured by TEM and were larger from its hydrodynamic sizes in water (1004 ± 134 nm), PBS (3373 ± 249 nm), serum free culture media (1735 ± 305 nm) and complete culture media (542 ± 108 nm). Surface reactivity of YO NPs with bovine serum albumin (BSA) was found significantly higher than for CeO and ZnO NPs. The displacement studies clearly suggested that adsorption to either BSA, filtered serum or serum free media was quite stable, and was dependent on whichever component interacted first with the YO NPs. Enzyme mimetic activity, like that of CeO NPs, was not detected for the NPs of YO or ZnO. Cell viability measured by MTT and neutral red uptake (NRU) assays suggested YO NPs were not toxic in human breast carcinoma MCF-7 and fibroblast HT-1080 cells up to the concentration of 200 μg/mL for a 24 h treatment period. Oxidative stress markers suggested YO NPs to be tolerably non-oxidative and biocompatible. Moreover, mitochondrial potential determined by JC-1 as well as lysosomal activity determined by lysotracker (LTR) remained un-affected and intact due to YO and CeO NPs whereas, as expected, were significantly induced by ZnO NPs. Hoechst-PI dual staining clearly suggested apoptotic potential of only ZnO NPs. With high surface reactivity and biocompatibility, NPs of YO could be a promising agent in the field of nanomedicine.
本研究旨在生成关于氧化钇纳米粒子（YO NPs）与抗氧化剂 CeO NPs 和促氧化剂 ZnO NPs 的生物反应的比较数据。通过 TEM 测量，发现 YO NPs 的尺寸在 35±10nm 范围内，而其在水中（1004±134nm）、PBS（3373±249nm）、无血清培养介质（1735±305nm）和完全培养介质（542±108nm）中的水动力尺寸更大。与 CeO 和 ZnO NPs 相比，YO NPs 与牛血清白蛋白（BSA）的表面反应性明显更高。取代研究清楚地表明，与 BSA、过滤后的血清或无血清培养基的吸附非常稳定，并且取决于与 YO NPs 首先相互作用的任何成分。酶模拟活性，如 CeO NPs 的酶模拟活性，未检测到 YO 或 ZnO NPs 的活性。MTT 和中性红摄取（NRU）测定法测量的细胞活力表明，在 24 小时处理期内，浓度高达 200μg/mL 时，YO NPs 对人乳腺癌 MCF-7 和成纤维细胞 HT-1080 细胞没有毒性。氧化应激标志物表明 YO NPs 是可耐受的非氧化性和生物相容性的。此外，JC-1 测定的线粒体电位以及溶酶体活性测定的溶酶体追踪器（LTR）由于 YO 和 CeO NPs 而保持不受影响和完整，而如预期的那样，ZnO NPs 则显著诱导了它们。Hoechst-PI 双重染色清楚地表明只有 ZnO NPs 具有潜在的凋亡作用。由于具有高表面反应性和生物相容性，YO NPs 可成为纳米医学领域的有前途的试剂。
