芬氯草醚对水稻生理、基因转录和丙草胺解毒能力的影响。

Effects of fenclorim on rice physiology, gene transcription and pretilachlor detoxification ability.

机构信息

College of Plant Protection, Hunan Agricultural University, Changsha, 410128, People's Republic of China.

Collaborative Innovation Center of Farmland Weeds Control, Hunan University of Humanities, Loudi, 417000, People's Republic of China.

出版信息

BMC Plant Biol. 2020 Mar 6;20(1):100. doi: 10.1186/s12870-020-2304-y.

BACKGROUND

Fenclorim (Fen) can effectively protect rice from pretilachlor (Pre) injury, but its effects on rice have not been formally evaluated; thus, the Fen mode of action for alleviating the phytotoxicity caused by Pre in rice is not clear. This study aimed to examine the biochemical and physiological effects of Fen on rice and to determine the changes induced by Fen at the transcriptome level.

RESULT

The chlorophyll content of rice plants was significantly affected by Pre but not by Fen. The activity of oxidative stress enzymes showed that Fen did not elicit any changes in oxidative stress; however, it reduced lipid peroxidation and oxidative damage induced by Pre. Fen did not affect the uptake of Pre but did affect its persistence in rice. In a transcriptome experiment, Fen upregulated genes in a detoxification pathway. Overall, 25 genes related to detoxification were identified, including P450, GST, and GT. Moreover, qRT-PCR analysis showed that four P450 genes, CYP71Y83, CYP71K14, CYP734A2 and CYP71D55, and two GST genes, GSTU16 and GSTF5, were upregulated by Fen and/or Pre.

CONCLUSION

Our work indicates that Fen acts in antioxidative defense in addition to enhancing the metabolism of herbicides in rice.

背景

芬克利姆（Fen）能有效保护水稻免受丙草胺（Pre）伤害，但尚未正式评估其对水稻的影响；因此，芬缓解丙草胺对水稻毒性的作用模式尚不清楚。本研究旨在探讨芬对水稻的生化和生理影响，并确定芬在转录组水平上诱导的变化。

结果

Pre 显著影响水稻的叶绿素含量，但芬没有。抗氧化应激酶的活性表明，芬不会引起氧化应激的任何变化，但会减少 Pre 诱导的脂质过氧化和氧化损伤。芬不影响 Pre 的吸收，但会影响其在水稻中的持久性。在转录组实验中，芬上调了解毒途径中的基因。总的来说，鉴定出了 25 个与解毒相关的基因，包括 P450、GST 和 GT。此外，qRT-PCR 分析表明，四个 P450 基因 CYP71Y83、CYP71K14、CYP734A2 和 CYP71D55 以及两个 GST 基因 GSTU16 和 GSTF5 被芬和/或 Pre 上调。