Department of Cranio-Maxillofacial and Oral Surgery, Center of Dental Medicine, University of Zurich, Zurich, Switzerland; Department of Oral Biotechnology and Bioengineering, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Clinic of Preventive Dentistry, Periodontology and Cariology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
J Endod. 2020 May;46(5):641-647. doi: 10.1016/j.joen.2020.01.016. Epub 2020 Mar 2.
Transforming growth factor beta 1 (TGF-β1) is a key morphogen in regenerative endodontics; yet, its location within the hard tissue phase of dentin and its availability in mature roots have not been fully elucidated.
Young mature (n = 8) and immature (n = 11) roots from sound premolars were obtained from 13 orthodontic patients aged 17 ± 1 and 12 ± 1 years, respectively. Roots were cleaned of organic remnants in 5% sodium hypochlorite. The width of the minor foramen was measured using a digital microscope. TGF-β1 distribution was assessed in 3 roots per group by immunostaining combined with confocal laser scanning microscopy. The root dentin of the remaining 13 roots was powdered and decalcified in 17% EDTA to determine the overall levels of hard tissue-embedded TGF-β1 by enzyme-linked immunosorbent assay. Data were compared between groups using the Student t test (α = .05).
The minor foramen was 168 ± 49 μm versus 557 ± 295 μm in mature compared with immature roots (P < .05). TGF-β1 was highly stainable toward the pulp space in both groups. It was clearly associated with peritubular dentin and apparently absent in nontubular outer dentin. TGF-β1 content was 115 ± 31 pg and 74 ± 35 pg/100 mg mature versus immature root dentin, respectively (P > .05).
TGF-β1 is deposited into the peritubular dentin. It should be possible to release this molecule in regenerative endodontic procedures from young mature roots as well as immature roots.
转化生长因子-β1（TGF-β1）是再生牙髓学中的关键形态发生因子；然而，其在牙本质硬组织阶段的位置及其在成熟根中的可用性尚未完全阐明。
从 13 名年龄分别为 17 ± 1 岁和 12 ± 1 岁的正畸患者中获得了 8 颗年轻成熟（n = 8）和 11 颗未成熟（n = 11）的前磨牙的根。用 5%次氯酸钠清除有机物残余物。用数字显微镜测量小根尖孔的宽度。通过免疫染色结合共聚焦激光扫描显微镜评估每组 3 根根 TGF-β1 的分布。用 17%EDTA 对其余 13 根根牙本质粉粹和脱钙，通过酶联免疫吸附试验确定硬组织中嵌入 TGF-β1 的总水平。使用学生 t 检验（α=.05）比较组间数据。
与成熟根相比，年轻成熟根的小根尖孔为 168 ± 49 μm，而不成熟根为 557 ± 295 μm（P <.05）。TGF-β1 在两组中均对牙髓空间高度可染色。它与管周牙本质明显相关，而在非管状外牙本质中明显不存在。TGF-β1 含量分别为 115 ± 31 pg 和 74 ± 35 pg/100 mg 成熟牙本质与不成熟牙本质（P >.05）。
TGF-β1 沉积在管周牙本质中。从年轻成熟的根以及不成熟的根中，在再生牙髓学程序中释放这种分子应该是可能的。
