Department of Pathology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Department of Pediatrics, University of Cincinnati, College of Medicine, Cincinnati, OH 45267, USA.
Sci Immunol. 2020 Mar 6;5(45). doi: 10.1126/sciimmunol.aay4209.
B cells in human food allergy have been studied predominantly in the blood. Little is known about IgE B cells or plasma cells in tissues exposed to dietary antigens. We characterized IgE clones in blood, stomach, duodenum, and esophagus of 19 peanut-allergic patients, using high-throughput DNA sequencing. IgE cells in allergic patients are enriched in stomach and duodenum, and have a plasma cell phenotype. Clonally related IgE and non-IgE-expressing cell frequencies in tissues suggest local isotype switching, including transitions between IgA and IgE isotypes. Highly similar antibody sequences specific for peanut allergen Ara h 2 are shared between patients, indicating that common immunoglobulin genetic rearrangements may contribute to pathogenesis. These data define the gastrointestinal tract as a reservoir of IgE B lineage cells in food allergy.
B 细胞在人类食物过敏中的作用主要在血液中进行了研究。对于暴露于饮食抗原的组织中的 IgE B 细胞或浆细胞知之甚少。我们使用高通量 DNA 测序技术对 19 名花生过敏患者的血液、胃、十二指肠和食管中的 IgE 克隆进行了表征。过敏患者的 IgE 细胞在胃和十二指肠中富集，并具有浆细胞表型。组织中克隆相关的 IgE 和非 IgE 表达细胞频率提示局部同种型转换，包括 IgA 和 IgE 同种型之间的转换。患者之间共享针对花生过敏原 Ara h 2 的高度相似的抗体序列，表明共同的免疫球蛋白基因重排可能有助于发病机制。这些数据将胃肠道定义为食物过敏中 IgE B 细胞系的储存库。
