Materials Chemistry Research Centre, Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, UK.
Department of Chemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
Nat Commun. 2020 Mar 5;11(1):1207. doi: 10.1038/s41467-020-15004-6.
The emergence of antibiotic resistant bacteria is a major threat to the practice of modern medicine. Photobactericidal agents have obtained significant attention as promising candidates to kill bacteria, and they have been extensively studied. However, to obtain photobactericidal activity, an intense white light source or UV-activation is usually required. Here we report a photobactericidal polymer containing crystal violet (CV) and thiolated gold nanocluster ([Au(Cys)]) activated at a low flux levels of white light. It was shown that the polymer encapsulated with CV do not have photobactericidal activity under white light illumination of an average 312 lux. However, encapsulation of [Au(Cys)] and CV into the polymer activates potent photobactericidal activity. The study of the photobactericidal mechanism shows that additional encapsulation of [Au(Cys)] into the CV treated polymer promotes redox reactions through generation of alternative electron transfer pathways, while it reduces photochemical reaction type-ІІ pathways resulting in promotion of hydrogen peroxide (HO) production.
抗生素耐药菌的出现是现代医学实践的主要威胁。光细菌杀灭剂作为一种有前途的杀菌候选物已经引起了广泛关注，并进行了广泛的研究。然而，为了获得光细菌杀灭活性，通常需要强烈的白光光源或 UV 激活。在这里，我们报告了一种含有结晶紫 (CV) 和巯基化金纳米簇 ([Au(Cys)]) 的光细菌杀灭聚合物，它可以在低通量的白光下激活。结果表明，在平均 312 lux 的白光照射下，包裹 CV 的聚合物没有光细菌杀灭活性。然而，将 [Au(Cys)] 和 CV 封装到聚合物中可以激活有效的光细菌杀灭活性。光细菌杀灭机制的研究表明，通过生成替代电子转移途径，将 [Au(Cys)] 额外封装到 CV 处理的聚合物中会促进氧化还原反应，同时减少光化学反应型-II 途径，从而促进过氧化氢 (HO) 的产生。
