School of Pharmaceutical Sciences, Jiangnan University, 1800 Lihu Ave, Wuxi, 21422, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Ave, Wuxi, 21422, China.
Curr Microbiol. 2020 Jul;77(7):1341-1347. doi: 10.1007/s00284-020-01934-3. Epub 2020 Mar 5.
A Gram-negative, motile, aerobic, and rod-shaped strain (MIC 1.5) was isolated from soil in Changguangxi national wetland park. Growth occurred at 20-45 °C, at pH 6.0-8.0, and at 0-4.0% NaCl. Based on 16S rRNA gene sequence analysis, strain MIC 1.5 was related to were identified as Luteimonas dalianensis CGMCC 1.12191 (95.3%), Luteimonas padinae DSM 101536 (94.5%), Luteimonas huabeiensis DSM 26429 (94.1%), and Luteimonas mephitis DSM 12574 (92.5%). The DNA-DNA relatedness values between strain MIC 1.5 , and these strains were well below 31%. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol. The DNA G+C content of strain MIC 1.5 was 66.3 mol%. Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC 1.5 and L. dalianensis CGMCC 1.12191 were 65.39% and 29.52%, respectively. The quinone was identified as Q-8. The major fatty acids were iso-C, iso-C 3OH, and iso-C 3OH and summed feature 3 (Cω7c and/or iso-C 2-OH). Based on the phylogenetic, physiological, and chemotaxonomic results, strain MIC 1.5 represents a novel species of the genus Luteimonas, for which the name Luteimonas cellulosilyticus sp. nov. is proposed. The type strain is MIC 1.5 (= KACC 19469 = CCTCC AB 2017256).
一株革兰氏阴性、运动性、需氧、杆状的菌株（MIC 1.5）是从长光希湿地国家公园的土壤中分离出来的。该菌在 20-45°C、pH 值 6.0-8.0 和 0-4.0%NaCl 条件下生长。根据 16S rRNA 基因序列分析，菌株 MIC 1.5 与 Luteimonas dalianensis CGMCC 1.12191（95.3%）、Luteimonas padinae DSM 101536（94.5%）、Luteimonas huabeiensis DSM 26429（94.1%）和 Luteimonas mephitis DSM 12574（92.5%）最为相似。菌株 MIC 1.5 与这些菌株之间的 DNA-DNA 同源性值均低于 31%。极性脂为磷脂酰乙醇胺、磷脂酰甘油和双磷脂酰甘油。菌株 MIC 1.5 的 DNA G+C 含量为 66.3mol%。菌株 MIC 1.5 与 L. dalianensis CGMCC 1.12191 的平均核苷酸同一性（ANI）和基因组到基因组距离（GGD）值分别为 65.39%和 29.52%。醌类物质为 Q-8。主要脂肪酸为 iso-C、iso-C 3OH 和 iso-C 3OH 和特征脂肪酸 3（Cω7c 和/或 iso-C 2-OH）。基于系统发育、生理和化学分类学的结果，菌株 MIC 1.5 代表了 Luteimonas 属的一个新种，命名为 Luteimonas cellulosilyticus sp. nov.。模式菌株为 MIC 1.5（=KACC 19469=CCTCC AB 2017256）。
