Department of General Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
Eur Rev Med Pharmacol Sci. 2020 Feb;24(4):1771-1777. doi: 10.26355/eurrev_202002_20354.
Some studies have confirmed that long non-coding ribonucleic acids (lncRNAs) played a vital role in the pathophysiology of various diseases, especially in oncogenesis and progression of tumors. Dysexpressed lncRNAs regulate different cellular processes, including proliferation, invasion, and apoptosis. The aim of this study was to explore the clinical significance and function of lncRNA MIR4435-2HG in colorectal cancer.
Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to detect the expression of lncRNA MIR4435-2HG. The Kaplan-Meier method was used to evaluate the overall survival and disease-free survival of patients with colorectal cancer. The cell proliferation was measured by Methyl thiazolyl tetrazolium (MTT) assay. The cell apoptotic rate was measured via flow cytometry method.
LncRNA MIR4435-2HG is a novel cancer-related lncRNA that was recently found to exhibit high expression in colorectal cancer. The dysregulation of lncRNA miR4435-2HG was significantly related to the tumor size (p<0.001), lymph node metastasis (p<0.001), and tumor node metastasis (TNM) staging (p=0.022). The patients with higher expression of lncRNA miR4435-2HG showed worse prognosis than those with low expression of lncRNA miR4435-2HG group. Besides, the downregulated lncRNA miR4435-2HG expression could repress cell proliferation and enhance cell apoptosis.
LncRNA MIR4435-2HG functions as an oncogene that promotes colorectal cancer progression, and likely represents a biomarker or therapeutic target of colorectal cancer.
一些研究证实，长非编码核糖核酸（lncRNA）在各种疾病的病理生理学中发挥着重要作用，尤其是在肿瘤的发生和进展中。表达失调的 lncRNA 调节着包括增殖、侵袭和凋亡在内的不同细胞过程。本研究旨在探讨 lncRNA MIR4435-2HG 在结直肠癌中的临床意义和功能。
采用实时定量聚合酶链反应（qRT-PCR）检测 lncRNA MIR4435-2HG 的表达。采用 Kaplan-Meier 法评估结直肠癌患者的总生存率和无病生存率。采用甲基噻唑基四唑（MTT）法检测细胞增殖。采用流式细胞术检测细胞凋亡率。
lncRNA MIR4435-2HG 是一种新的癌症相关 lncRNA，最近发现其在结直肠癌中表达升高。lncRNA miR4435-2HG 的失调与肿瘤大小（p<0.001）、淋巴结转移（p<0.001）和肿瘤淋巴结转移（TNM）分期（p=0.022）显著相关。lncRNA miR4435-2HG 高表达的患者预后比 lncRNA miR4435-2HG 低表达的患者差。此外，下调 lncRNA miR4435-2HG 的表达可抑制细胞增殖并增强细胞凋亡。
lncRNA MIR4435-2HG 作为一种癌基因促进结直肠癌的进展，可能代表结直肠癌的生物标志物或治疗靶点。
