长非编码 RNA MIR4435-2HG 通过招募 FLOT2 上的 miR-802 促进黑色素瘤进展。

Long non-coding RNA MIR4435-2HG recruits miR-802 from FLOT2 to promote melanoma progression.

机构信息

Department of Dermatology and Venereology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China.

出版信息

Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2616-2624. doi: 10.26355/eurrev_202003_20530.

DOI:10.26355/eurrev_202003_20530

PMID:32196611

Abstract

OBJECTIVE

The regulatory mechanism of lncRNA MIR4435-2HG has been extensively investigated in human cancers other than melanoma. This study aims to elucidate the role of lncRNA MIR4435-2HG in melanoma.

MATERIAL AND METHODS

The mRNA expression was detected by RT-qPCR. MTT assay, Transwell assay and Dual-Luciferase reporter assay were used to investigate the regulatory mechanism of lncRNA MIR4435-2HG.

RESULTS

Upregulation of lncRNA MIR4435-2HG was identified in melanoma and promoted melanoma cell proliferation, migration and invasion. In addition, lncRNA MIR4435-2HG serves as the ceRNA of miR-802. MiR-802 inhibited melanoma progression by downregulating lncRNA MIR4435-2HG. Besides, miR-802 directly targets FLOT2. And knockdown of FLOT2 restrained the progression of melanoma by downregulating lncRNA MIR4435-2HG and upregulating miR-802.

CONCLUSIONS

LncRNA MIR4435-2HG promotes cell proliferation, migration and invasion in melanoma by sponging miR-802 and upregulating FLOT2.

摘要

目的

lncRNA MIR4435-2HG 的调控机制已在黑色素瘤以外的人类癌症中得到广泛研究。本研究旨在阐明 lncRNA MIR4435-2HG 在黑色素瘤中的作用。

材料与方法

采用 RT-qPCR 检测 mRNA 表达。MTT assay、Transwell assay 和 Dual-Luciferase reporter assay 用于研究 lncRNA MIR4435-2HG 的调控机制。

结果

lncRNA MIR4435-2HG 在黑色素瘤中上调，并促进黑色素瘤细胞增殖、迁移和侵袭。此外，lncRNA MIR4435-2HG 作为 miR-802 的 ceRNA。miR-802 通过下调 lncRNA MIR4435-2HG 抑制黑色素瘤进展。此外，miR-802 直接靶向 FLOT2。FLOT2 的敲低通过下调 lncRNA MIR4435-2HG 和上调 miR-802 来抑制黑色素瘤的进展。