鼠伤寒沙门氏菌中组氨酸转运基因的精细结构图谱。
Fine-structure map of the histidine transport genes in Salmonella typhimurium.
作者信息
Ames G F, Noel K D, Taber H, Spudich E N, Nikaido K, Afong J
出版信息
J Bacteriol. 1977 Mar;129(3):1289-97. doi: 10.1128/jb.129.3.1289-1297.1977.
Abstract
Afine-structure genetic map of the histidine transport region of the Salmonella typhimurium chromosome was constructed. Twenty-five deletion mutants were isolated and used for dividing the hisJ and hisP genes into 8 and 13 regions respectively. A total of 308 mutations, spontaneous and mutagen induced, have been placed in these regions by deletion mapping. The histidine transport operon is presumed to be constituted of genes dhuA, hisJ, and hisP, and the regulation of the hosP and hisJ genes by dhuA is discussed. The orientation of this operon relative to purF has been established by three-point crosses as being: purF duhA hisJ hisP.
摘要
构建了鼠伤寒沙门氏菌染色体组氨酸转运区域的精细结构遗传图谱。分离出25个缺失突变体，并分别用于将hisJ和hisP基因划分为8个和13个区域。通过缺失作图，已将总共308个自发突变和诱变诱导的突变定位到这些区域。推测组氨酸转运操纵子由dhuA、hisJ和hisP基因组成，并讨论了dhuA对hosP和hisJ基因的调控。通过三点杂交确定了该操纵子相对于purF的方向为：purF dhuA hisJ hisP。
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