沙门氏菌组氨酸操纵子-启动子的精细结构图谱。
A fine structure map of the salmonella histidine operator-promoter.
作者信息
Ely B, Fankhauser D B, Hartman P E
出版信息
Genetics. 1974 Oct;78(2):607-31. doi: 10.1093/genetics/78.2.607.
Abstract
Over 100 regulatory mutations linked to the histidine (his) operon of S. typhimurium have been isolated. They all map in a region estimated to be several hundred base pairs in length located at one end of the his operon ("the hisO region"). The mutations are located at sixteen recombinationally separable sites or are deletions encompassing several sites. Data obtained from pairs of reciprocal three-point tests show that "constitutive" (high enzyme levels) and "promoter-like" (low enzyme levels) hisO mutations are interspersed on the genetic map. In a few crosses, recombination was not observed to occur between markers shown to occupy different sites based on behavior in other recombination tests.
摘要
已分离出100多个与鼠伤寒沙门氏菌组氨酸（his）操纵子相关的调控突变。它们都定位在一个估计长度为几百个碱基对的区域，该区域位于his操纵子的一端（“hisO区域”）。这些突变位于16个重组可分离位点，或者是包含几个位点的缺失。从成对的三点互交试验获得的数据表明，“组成型”（高酶水平）和“启动子样”（低酶水平）hisO突变在遗传图谱上是交错分布的。在少数杂交中，基于其他重组试验中的行为显示占据不同位点的标记之间未观察到重组发生。
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