The Key Laboratory of Environment and Health, School of Public Health, Fujian Medical University, Fuzhou, China.
Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou, China.
Methods Mol Biol. 2021;2326:123-141. doi: 10.1007/978-1-0716-1514-0_9.
Epigenetics is one of the most rapidly expanding fields in biology, which plays important roles in environmental pollutant-induced neurotoxicity. Analyses of epigenetic modification is of great significance in providing more accurate information for the risk assessment and management of harmful factors. However, few studies have systematically summarized the analysis and detection methods for epigenetic modification. In this chapter, we summarized several popular methods for analyses of epigenetic modifications, including Methylated DNA Immunoprecipitation Sequencing (MeDIP-Seq) for genome-wide DNA methylation analyses, Quantitative Methylation Specific PCR (qMSP) for genome-specific DNA methylation analyses, methylated RNA immunoprecipitation sequencing (MeRIP-seq) for genome-wide RNA methylation analyses, MeRIP-qPCR for genome-specific RNA methylation analyses, qRT-PCR for the non-coding RNA, and western blot for the histone modification analyses. It could be helpful to the research about environmental epigenetic toxicology.
表观遗传学是生物学中发展最快的领域之一，在环境污染物诱导的神经毒性中起着重要作用。对表观遗传修饰的分析在为有害因素的风险评估和管理提供更准确的信息方面具有重要意义。然而，很少有研究系统地总结了表观遗传修饰的分析和检测方法。在本章中，我们总结了几种常用的表观遗传修饰分析方法，包括用于全基因组 DNA 甲基化分析的甲基化 DNA 免疫沉淀测序（MeDIP-Seq）、用于基因组特异性 DNA 甲基化分析的定量甲基化特异性 PCR（qMSP）、用于全基因组 RNA 甲基化分析的甲基化 RNA 免疫沉淀测序（MeRIP-seq）、用于基因组特异性 RNA 甲基化分析的 MeRIP-qPCR、用于非编码 RNA 的 qRT-PCR 和用于组蛋白修饰分析的 Western blot。这可能有助于环境表观毒理学的研究。
