Dept. of Orthopedics, Affiliated Hospital of Youjiang Medical Univ. for Nationalities, Baise, China.
J Food Sci. 2020 Apr;85(4):1302-1306. doi: 10.1111/1750-3841.14986. Epub 2020 Mar 6.
The effects and mechanisms of soybean isoflavone on osteoblast (OB) proliferation in vitro were investigated. Fifty female Wistar rats were randomly divided into five groups with 10 rats in each group. Rat OBs were separated and cultured. The first generation of OBs cultured for 48 hr at various concentrations of isoflavone were set as the experimental groups, the OBs exposed to estradiol (E ) culture were considered as positive control group. The biological characterization of OBs was investigated by phase contrast microscopy and alkaline phosphatase (ALP) histochemistry. The concentrations of interleukin (IL-1), osteoprotegerin (OPG), transforming growth factor (TGF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and vascular endothelial growth factor (VEGF) in isoflavone culture solutions were determined. Proliferation rate of OBs was increased in experimental group comparing that in the blank group. ALP activity in experimental group was higher than that in blank group. No significant differences of ALP activity were observed between E culture group and isoflavone group at concentrations of 10 and 10 mM (P > 0.05). Furthermore, in the experimental groups at low isoflavone concentrations, the concentrations of OPG, TGF, and VEGF were increased and positively correlated with OB proliferation. However, the concentrations of IL-1, GM-CSF were decreased at higher concentration of isoflavone and were negatively correlated with OB proliferation. Soybean isoflavone could promote the growth and proliferation of rat OB, it might act as the stimulator of OPG, TGF, and VEGF pathway, and the inhibitor of IL-1, GM-CSF pathway as well.
研究了大豆异黄酮对体外成骨细胞（OB）增殖的作用及机制。将 50 只雌性 Wistar 大鼠随机分为 5 组，每组 10 只。分离培养大鼠 OB。将培养 48 小时的不同浓度异黄酮第一代 OB 设为实验组，将暴露于雌二醇（E）培养的 OB 设为阳性对照组。通过相差显微镜和碱性磷酸酶（ALP）组织化学观察 OB 的生物学特征。测定异黄酮培养溶液中白细胞介素（IL-1）、护骨素（OPG）、转化生长因子（TGF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）和血管内皮生长因子（VEGF）的浓度。与空白组相比，实验组 OB 的增殖率增加。实验组的 ALP 活性高于空白组。在浓度为 10 和 10 mM 时，E 培养组和异黄酮组的 ALP 活性无显著差异（P>0.05）。此外，在低浓度异黄酮实验组中，OPG、TGF 和 VEGF 的浓度增加，与 OB 增殖呈正相关。然而，在较高浓度的异黄酮下，IL-1、GM-CSF 的浓度降低，与 OB 增殖呈负相关。大豆异黄酮能促进大鼠 OB 的生长和增殖，可能作为 OPG、TGF 和 VEGF 途径的刺激剂，以及 IL-1、GM-CSF 途径的抑制剂。
