Zhang Chen Lei, Hui Jian Rong, Li Yong Feng, Zhang Rui, Lü Yao, Li Yan Jiao
School of Acupuncture and Massage, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China.
School of Acupuncture and Massage, Shaanxi University of Chinese Medicine, Xianyang 712046, Shaanxi Province, China; The First Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, Shaanxi Province.
Zhen Ci Yan Jiu. 2020 Feb 25;45(2):122-7. doi: 10.13702/j.1000-0607.1904846.
To investigate the effect of Tongli Shuji acupuncture on the behavioristics, the percentage of cerebral infarct volume, and the expression of Jagged2 and Notch2 proteins in the hippocampus of the ischemic hemisphere in rats with permanent ischemia of the right middle cerebral artery, as well as the mechanism of Tongli Shuji acupuncture in improving ischemic cerebrovascular disease.
Sprague-Dawley rats were randomly divided into sham-operation group, model control group, medication group, routine acupuncture group, and Tongli Shuji group, with 10 rats in each group. The suture method was used to establish a model of permanent ischemia of the right middle cerebral artery. The rats in the medication group were given Citicoline (0.4 mg/kg) by gavage, those in the routine acupuncture group were given acupuncture at"Baihui"(GV20) and"Dazhui" (GV14) 15 minutes per day, and those in the Tongli Shuji group were given acupuncture at "Zhengying "(GB17), "Tianjing"(SJ10), and"Huantiao"(GB30) at the left side. The neurological deficit score was determined on days 1, 7, 14, 21 and 28 after surgery, the percentage of cerebral infarct volume was measured after treatment, and Western blot was used to measure the protein expression of Jagged2 and Notch2 in the right hippocampal tissue.
Compared with the sham-operation group, the model control group had significant increases in the neurological deficit score on day 1 after surgery (<0.001). Compared with the model control, medication and routine acupuncture groups, the Tongli Shuji group had significant reductions in the neurological deficit score on day 21 after surgery (<0.01). Compared with the sham-operation group, the model control group had a significant increase in the percentage of cerebral infarct volume(<0.05)．The Tongli Shuji group had a significant reduction in the percentage of cerebral infarct volume compared with the medication groups (<0.05). Compared with the model control group, the routine acupuncture group had a significant increase in the expression of hippocapmal Jagged2(<0.01)．Compared with the model control, medication and routine acupuncture groups, the Tongli Shuji group had significant increases in the expression of hippocampal Jagged2 and Notch2 (<0.01).
Acupuncture can improve the behavioral manifestations of neurological deficit in rats with permanent ischemia of the right middle cerebral artery. Tongli Shuji acupuncture can significantly up-regulate the protein expression of Jagged2 and Notch2 in the hippocampal tissue of the ischemic region, possibly by activating the Notch signaling pathway to exert a neuroprotective effect.
观察通里枢机针法对大脑中动脉永久性缺血大鼠行为学、脑梗死体积百分比及缺血侧海马区锯齿状蛋白2（Jagged2）和Notch2蛋白表达的影响，探讨通里枢机针法改善缺血性脑血管病的作用机制。
将Sprague-Dawley大鼠随机分为假手术组、模型对照组、药物组、常规针刺组和通里枢机组，每组10只。采用线栓法制备大脑中动脉永久性缺血模型。药物组给予胞磷胆碱（0.4mg/kg）灌胃；常规针刺组每日针刺“百会”（GV20）、“大椎”（GV14）15分钟；通里枢机组针刺左侧“正营”（GB17）、“天井”（SJ10）、“环跳”（GB30）。于术后1、7、14、21、28天进行神经功能缺损评分，治疗后测量脑梗死体积百分比，采用蛋白质免疫印迹法检测右侧海马组织中Jagged2和Notch2蛋白表达。
与假手术组比较，模型对照组术后1天神经功能缺损评分显著升高（<0.001）。与模型对照组、药物组、常规针刺组比较，通里枢机组术后21天神经功能缺损评分显著降低（<0.01）。与假手术组比较，模型对照组脑梗死体积百分比显著升高（<0.05）。与药物组比较，通里枢机组脑梗死体积百分比显著降低（<0.05）。与模型对照组比较，常规针刺组海马Jagged2表达显著升高（<0.01）。与模型对照组、药物组、常规针刺组比较，通里枢机组海马Jagged2和Notch2表达显著升高（<0.01）。
针刺可改善大脑中动脉永久性缺血大鼠神经功能缺损行为学表现。通里枢机针法可显著上调缺血区海马组织Jagged2和Notch2蛋白表达，可能是通过激活Notch信号通路发挥神经保护作用。
