Wei Jian, Xie Chun-Zhu, Yuan Qing
Clinical Medical College of Acupuncture, Moxibustion and Rehabilitation, Guangzhou University of CM, Guangzhou 510405, Guangdong Province, China.
Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of CM.
Zhongguo Zhen Jiu. 2019 Jul 12;39(7):748-54. doi: 10.13703/j.0255-2930.2019.07.018.
To explore the effects on the recovery of the motor and cognitive functions of the rats with permanent middle cerebral artery occlusion (pMCAO) after treated with 's three-needle acupuncture at head acupoints combined with rota-rod training.
A total of 38 male SD rats were randomized into 3 groups, named a sham-operation group (11 rats), a model group (13 rats) and a treatment group (14 rats). The electrocoagulation method was adopted to establish the model of pMCAO on the right cerebrum. Starting from the 1st day after successful modeling, acupuncture was applied to the "three points of intelligence", the "three points of temporal area" and the "three points of brain". Additionally, the rota-rod training was used. Acupuncture was given once a day and the training was three times a day. In the sham-operation group and the model group, empty grasp fixation was performed when acupuncture was applied in the treatment group, and there was no intervention at the rest of the time. There was 1 day of interval after consecutive 6 days of intervention. Totally, the intervention was for 3 weeks. After modeling, the brain section was collected from 3 rats of each group on the 1st day and was stained with TTC to observe the condition of cerebral ischemia. From day 1 to 7, the neurological function score was evaluated. The footprint analysis and rota-rod test were performed on day 1, 7, 14 and 21. The Morris water maze test was performed from day 22 to 26.
Compared with the sham-operation group, cerebral ischemia presented obviously, the score of neurological function was increased, the back front distances on the left were increased on day 1, 7 and 14 separately, the revolutions per minute (RPM) of the rota-rod were reduced at each of the above 4 time points, the latency of navigation trial was increased and the movement time percentage in Q3 quadrant of spatial probe trial was reduced in the model group (<0.01, <0.05). Compared with the model group, the area of cerebral ischemia was not obviously different (>0.05), the score of neurological function was reduced on day 6, the back front distance on the left was reduced on day 14, RPM of the rota-rod were increased on day 14 and 21, the latency of navigation trial were reduced from day 23 to 25 and the movement time percentage in Q3 quadrant of spatial probe trial was increased in the treatment group (<0.01, <0.05).
's three-needle acupuncture at head acupoints combined with rota-rod training improve the behavioral performance of pMCAO rats and promote the recovery of motor and cognitive functions.
探讨头穴“三才针法”结合转棒训练对永久性大脑中动脉闭塞（pMCAO）大鼠运动和认知功能恢复的影响。
将38只雄性SD大鼠随机分为3组，即假手术组（11只）、模型组（13只）和治疗组（14只）。采用电凝法建立右侧大脑pMCAO模型。造模成功后第1天开始，对头穴“智三针”“颞三针”“脑三针”进行针刺，并结合转棒训练。针刺每日1次，训练每日3次。假手术组和模型组在治疗组针刺时进行空抓固定，其余时间不干预。连续干预6天后间隔1天，共干预3周。造模后第1天每组取3只大鼠取脑切片，进行TTC染色观察脑缺血情况。从第1天至第7天评估神经功能评分。在第1、7、14和21天进行足迹分析和转棒试验。从第22天至第26天进行Morris水迷宫试验。
与假手术组比较，模型组脑缺血明显，神经功能评分升高，在第1、7和14天左侧的前后距离分别增加，在上述4个时间点转棒每分钟转速（RPM）均降低，定位航行试验潜伏期延长，空间探索试验在第3象限的运动时间百分比降低（P<0.01，P<0.05）。与模型组比较，治疗组脑缺血面积无明显差异（P>0.05），在第6天神经功能评分降低，在第14天左侧前后距离缩短，在第14和21天转棒RPM升高，从第23天至第25天定位航行试验潜伏期缩短，空间探索试验在第3象限的运动时间百分比增加（P<0.01，P<0.05）。
头穴“三才针法”结合转棒训练可改善pMCAO大鼠的行为学表现，促进运动和认知功能恢复。
