含有修饰核苷Q的大肠杆菌前体tRNA的分离
Isolation of Escherichia coli precursor tRNAs containing modified nucleoside Q.
作者信息
Vögeli G, Stewart T S, McCutchan T, Söll D
出版信息
J Biol Chem. 1977 Apr 10;252(7):2311-8.
PMID:321455
Abstract
Affinity chromatography based on the complex formation of the modified nucleoside Q with boronic acid has been applied to the isolation of specific tRNA precursors containing this modified nucleoside. When [32P]RNA isolated from an Escherichia coli strain containing a thermolabile ribonuclease P was chromatographed on dihydroxyboryl-substituted cellulose, the precursors for asparagine, aspartate, histidine, and tyrosine tRNA were specifically retained. All precursors were monomeric. The nucleotide sequences of four asparagine tRNA precursors were determined.
摘要
基于修饰核苷Q与硼酸形成复合物的亲和色谱法已应用于含有这种修饰核苷的特定tRNA前体的分离。当从含有热不稳定核糖核酸酶P的大肠杆菌菌株中分离出的[32P]RNA在二羟基硼基取代的纤维素上进行色谱分析时，天冬酰胺、天冬氨酸、组氨酸和酪氨酸tRNA的前体被特异性保留。所有前体均为单体。测定了四种天冬酰胺tRNA前体的核苷酸序列。
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