Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 106, Taiwan.
Institute of Biochemical and Biomedical Engineering, Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei, 106, Taiwan.
Anal Chim Acta. 2020 Apr 15;1106:168-175. doi: 10.1016/j.aca.2020.01.065. Epub 2020 Jan 30.
A ratiometric electrochemical molecular sensing platform for real-time quantification of extracellular hypochlorous acid (HClO) production has been developed based on a latent electrochemical probe aminoferrocene thiocarbamate (AFTC 3). The substrate AFTC consist of a masked redox reporter amino ferrocene (AF 4) linked with a dimethylthiocarbamate trigger via hydroxyl benzyl alcohol. The conceptual idea behind the probe design is based on a specific chemical interaction between HClO and dimethylthiocarbamate, which allows only HClO to unmask the probe to releases AF. The scheme was manipulated to establish a highly selective (in presence of various reactive oxygen species, anions and other biological interfering species) and sensitive (detection limit 75 nM) sensing platform not only in lab samples but also in real samples (food samples, and live cells). Real-time in situ quantification platform was developed to profile HClO productions in macrophages, and it did so with great consistency.
基于潜伏电化学探针氨基二茂铁硫代氨基甲酸酯（AFTC 3），开发了一种用于实时定量检测细胞外次氯酸（HClO）产生的比率型电化学分子传感平台。该底物 AFTC 由一个掩蔽的氧化还原报告基团氨基二茂铁（AF 4）通过羟基苯甲醇与二甲基硫代氨基甲酸酯触发基团相连。探针设计背后的概念是基于 HClO 和二甲基硫代氨基甲酸酯之间的特定化学反应，这使得只有 HClO 能够揭开探针，释放出 AF。该方案被操纵以建立一个高度选择性（在存在各种活性氧物质、阴离子和其他生物干扰物质的情况下）和灵敏（检测限为 75 nM）的传感平台，不仅在实验室样本中，而且在真实样本（食品样本和活细胞）中也是如此。还开发了实时原位定量平台来分析巨噬细胞中的 HClO 产生情况，其结果非常一致。
