School of Kinesiology, Shanghai University of Sport, Shanghai, 200438, China.
Ministry of Science and Education, Anhui Provincial Hospital of Integrated Chinese and Western Medicine (The Third Affiliated Hospital of Anhui University of Chinese Medicine), No. 45, Shihe Road, Wulidun Subdistrict, Shushan District, Hefei, 230061, Anhui Province, China.
J Bone Miner Metab. 2020 Jul;38(4):491-500. doi: 10.1007/s00774-020-01092-3. Epub 2020 Mar 7.
To investigate the effect of different frequencies of whole body vibration (WBV) on articular cartilage of early knee osteoarthritis (OA) rats and determine whether WBV would influence the pathway of hypoxia-inducible factor-2α (HIF-2α) regulation-related genes after 8 weeks of treatment.
Forty 8-week-old OA rats were divided into five groups: sham control (SC); high frequency 60 Hz (HV); high frequency 40 Hz (HV); middle frequency 20 Hz (MV) and low frequency 10 Hz (LV). WBV (0.3 g) treatment was given 40 min/day and 5 days/week. After 8 weeks, rats were killed and knees were harvested. OA grading score: Osteoarthritis Research Society International (OARSI), and the expression of related genes: interleukin-1β (IL-1β), HIF-2α, matrix metalloproteinases-13 (MMP-13), and collagen type II alpha 1 (COL2A1), at both mRNA and protein levels were analyzed.
After 8 weeks of WBV, our data showed that lower frequency (10 Hz) was more effective than the higher ones, yet they all suggested that WBV alleviates the erosion of knee articular cartilage in early OA. The expression of IL-1β, HIF-2α and MMP-13 decreased with frequency and reached the lowest level at 10 Hz, the expression of COL2A1 increased with frequency and reached the highest level at 10 Hz.
This study demonstrates that WBV could alleviate the degeneration of knee joints in an early OA rat model. WBV regulates related gene expression at both mRNA and protein levels. HIF-2α could be a therapeutic target. The effect of WBV is frequency dependent; the lower frequency shows better effects.
本研究旨在探讨不同频率全身振动（WBV）对早期膝骨关节炎（OA）大鼠关节软骨的影响，并确定 WBV 是否会影响缺氧诱导因子-2α（HIF-2α）调节相关基因的通路。
将 40 只 8 周龄 OA 大鼠分为 5 组：假手术对照组（SC）；高频 60Hz（HV）组；高频 40Hz（HV）组；中频 20Hz（MV）组和低频 10Hz（LV）组。WBV（0.3g）治疗每天 40 分钟，每周 5 天。8 周后处死大鼠，取膝关节。采用骨关节炎研究协会国际评分（OARSI）评估 OA 分级评分，采用实时定量 PCR 和蛋白质印迹法检测相关基因白细胞介素 1β（IL-1β）、HIF-2α、基质金属蛋白酶 13（MMP-13）和 I 型胶原α 1 链（COL2A1）的表达。
经过 8 周的 WBV 治疗，我们的数据表明低频（10Hz）比高频更有效，但都表明 WBV 可减轻早期 OA 膝关节软骨的侵蚀。随着频率的增加，IL-1β、HIF-2α 和 MMP-13 的表达降低，在 10Hz 时达到最低水平，COL2A1 的表达随频率增加而增加，在 10Hz 时达到最高水平。
本研究表明，WBV 可减轻早期 OA 大鼠模型膝关节的退变。WBV 调节相关基因在 mRNA 和蛋白质水平的表达。HIF-2α 可能是一个治疗靶点。WBV 的作用与频率有关，低频效果更好。
