Karoum F, Chuang L W, Mosnaim A D, Staub R A, Wyatt R J
J Chromatogr Sci. 1983 Dec;21(12):546-50. doi: 10.1093/chromsci/21.12.546.
A rapid and reliable mass-fragmentographic method for assay of plasma and cerebrospinal fluid (CSF) concentrations of free and conjugated phenylacetic acid (PAA) is described. The method is used to compare plasma and CSF concentrations of PAA in humans and monkeys. Both packed and capillary columns are used. In humans approximately 45% of total plasma PAA is conjugated in contrast to approximately 60% in monkeys. Both free and conjugated PAA concentrations tend to be higher in monkeys than in humans. Plasma mean concentration of total PAA in humans and monkeys are, respectively, 459.1 and 838 ng/ml. Approximately 55 and 25% of total PAA in the CSF are conjugated in humans and monkeys, respectively. Total PAA mean concentrations in human and monkey CSF are 41.6 and 84.2 ng/ml. Because over 90% of total urine PAA in humans is conjugated, it is concluded that over 50% of urine phenylacetylglutamine may be derived from kidney conjugation of free plasma PAA and/or from the kidney's preferential filtration of conjugated PAA as contrasted with free PAA.
本文描述了一种快速可靠的质量碎片谱法，用于测定血浆和脑脊液（CSF）中游离和结合型苯乙酸（PAA）的浓度。该方法用于比较人和猴血浆及脑脊液中PAA的浓度。使用了填充柱和毛细管柱。在人类中，约45%的血浆总PAA是结合型的，而在猴中约为60%。猴中游离和结合型PAA的浓度往往高于人类。人类和猴血浆中总PAA的平均浓度分别为459.1和838 ng/ml。在人类和猴的脑脊液中，分别约55%和25%的总PAA是结合型的。人类和猴脑脊液中总PAA的平均浓度分别为41.6和84.2 ng/ml。由于人类尿液中超过90%的总PAA是结合型的，因此得出结论，超过50%的尿苯乙酰谷氨酰胺可能来自游离血浆PAA的肾脏结合和/或来自肾脏对结合型PAA相对于游离PAA的优先过滤。
