Moon D C, Kelley J A
Division of Cancer Treatment, National Cancer Institute, NIH, Bethesda, Maryland 20892.
Biomed Environ Mass Spectrom. 1988 Sep;17(3):229-37. doi: 10.1002/bms.1200170312.
A simple and efficient procedure for desalting samples prior to fast atom bombardment mass spectrometry utilizes low-pressure reverse-phase chromatography with disposable high-capacity mini-columns. Microscale (0.1-10 mumol) sample purification with high analyte recovery is possible for nucleosides, nucleotides and peptides. Salt removal has been evaluated both by conductivity and mass spectral methods and is greater than 99% for the model compounds cytidine, adenosine and AMP, even when they are contaminated with a 10 molar excess of NaCl. This procedure is also applicable to a range of small peptides as demonstrated with pyro-Glu-His-Gly and methionine enkephalin.
一种用于在快速原子轰击质谱分析之前对样品进行脱盐的简单高效方法，采用带有一次性高容量微型柱的低压反相色谱法。对于核苷、核苷酸和肽，可以实现微尺度（0.1 - 10微摩尔）的样品纯化且分析物回收率高。已通过电导率和质谱方法对脱盐效果进行评估，对于模型化合物胞苷、腺苷和AMP，即使它们被10摩尔过量的NaCl污染，脱盐率也大于99%。如焦谷氨酸 - 组氨酸 - 甘氨酸和甲硫氨酸脑啡肽所示，该方法也适用于一系列小肽。
