Jacques M, Roy G, Mittal K R
Groupe de recherche sur les maladies infectieuses du porc, Faculté de médecine vétérinaire, Université de Montréal, Québec, Canada.
Can J Microbiol. 1988 Sep;34(9):1046-9. doi: 10.1139/m88-184.
A total of 26 isolates of Actinobacillus pleuropneumoniae were tested for their ability to agglutinate erythrocytes of different origins. Seven different hemagglutination patterns were found. Ten (38%) isolates did not agglutinate any of the erythrocytes tested. The remaining 16 (62%) isolates agglutinated human erythrocytes, and among these, 12 also agglutinated rat, cat, dog, guinea pig, or bovine erythrocytes. No correlation was found between the seven different hemagglutination patterns observed and the serotypes. Hemagglutination activity was destroyed by heating at 100 degrees C as well as by formaldehyde treatment, but was not affected by heating at 60 degrees C, by treatment with trypsin or pronase, or by homogenization of bacterial cells. No fimbriae were observed on examination of bacterial cells negatively stained with phosphotungstate using electron microscopy. Hydrophobic surface properties of the isolates were evaluated. All the isolates appear to possess a hydrophilic cell surface. The present study provides evidence that certain isolates of A. pleuropneumoniae possess hemagglutinating properties which do not appear to be mediated by fimbriae or to involve hydrophobic interactions.
共对26株胸膜肺炎放线杆菌分离株凝集不同来源红细胞的能力进行了检测。发现了7种不同的血凝模式。10株（38%）分离株不凝集任何所检测的红细胞。其余16株（62%）分离株凝集人红细胞，其中12株还凝集大鼠、猫、狗、豚鼠或牛的红细胞。观察到的7种不同血凝模式与血清型之间未发现相关性。血凝活性在100℃加热以及甲醛处理后被破坏，但不受60℃加热、胰蛋白酶或链霉蛋白酶处理或细菌细胞匀浆的影响。使用磷钨酸盐负染细菌细胞，通过电子显微镜检查未观察到菌毛。对分离株的疏水表面特性进行了评估。所有分离株似乎都具有亲水性细胞表面。本研究提供了证据，表明某些胸膜肺炎放线杆菌分离株具有血凝特性，这些特性似乎不是由菌毛介导的，也不涉及疏水相互作用。
