Mei Zhixiong, Huang Baoqin, Qian Xialiu, Zhang Yuan, Teng Benqi
Department of Obstetrics The Third Affiliated Hospital Sun Yat-sen University Guangzhou China.
Food Sci Nutr. 2020 Jan 10;8(2):820-829. doi: 10.1002/fsn3.1342. eCollection 2020 Feb.
To explain gastrodin improved cell apoptosis induced by preeclampsia in vivo and in vitro study. The PE and normal rats were injected with normal saline (Model), low-dose gastrodin (Gas-L), medium-dose gastrodin (Gas-M), and high-dose gastrodin (Gas-H) groups at 50, 100, or 200 mg/kg per day. The rat blood pressure and 24-hr urine protein level were measured at pregnant days 10, 16, and 20. Evaluating pathology by H&E staining, the cell apoptosis by TUNEL, and MyD88 and NF-κB (p65) proteins by IHC assay using H/R to simulate PE cell model. Measuring cell proliferation, apoptosis, and MyD88 and NF-κB (p65) protein expression by MTT, flow cytometry, and WB assay. The SBP, DBP, and 24-hr urine protein levels were significantly different in PE rats ( < .05). The SBP, DBP, and 24-hr urine protein levels were significantly improved ( < .05) in vivo and in vitro. The positive apoptosis cells and apoptosis rate were significantly increased with MyD88 and NF-κB (p65) proteins upregulation ( < .05). The positive apoptosis cells and apoptosis rate were significantly decreased with MyD88 and NF-κB (p65) proteins depressing in gastrodin-treated groups with dose-dependent ( < .05). Gastrodin improves PE-induced cell apoptosis and pathology changed via MyD88/NF-κB pathway in vitro and in vivo study.
为解释天麻素在体内和体外研究中改善子痫前期诱导的细胞凋亡的作用。将PE大鼠和正常大鼠分为模型组（注射生理盐水）、低剂量天麻素组（Gas-L，每天50mg/kg）、中剂量天麻素组（Gas-M，每天100mg/kg）和高剂量天麻素组（Gas-H，每天200mg/kg）。在妊娠第10、16和20天测量大鼠血压和24小时尿蛋白水平。通过苏木精-伊红（H&E）染色评估病理学，通过TUNEL法检测细胞凋亡，通过免疫组化（IHC）法检测MyD88和NF-κB（p65）蛋白，使用缺氧/复氧（H/R）模拟PE细胞模型。通过MTT法、流式细胞术和蛋白质免疫印迹（WB）法测量细胞增殖、凋亡以及MyD88和NF-κB（p65）蛋白表达。PE大鼠的收缩压（SBP）、舒张压（DBP）和24小时尿蛋白水平有显著差异（P<0.05）。天麻素在体内和体外均能显著改善SBP、DBP和24小时尿蛋白水平（P<0.05）。随着MyD88和NF-κB（p65）蛋白上调，阳性凋亡细胞和凋亡率显著增加（P<0.05）。在天麻素治疗组中，随着MyD88和NF-κB（p65）蛋白表达受到抑制，阳性凋亡细胞和凋亡率显著降低，且呈剂量依赖性（P<0.05）。在体内和体外研究中，天麻素通过MyD88/NF-κB途径改善子痫前期诱导的细胞凋亡和病理变化。
