Department of Experimental Pharmacology and Toxicology, School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, China.
Department of Pathology, The Third Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China.
Biomed Res Int. 2020 Feb 21;2020:9396512. doi: 10.1155/2020/9396512. eCollection 2020.
. Gastric cancer, one of the most common malignant tumors worldwide, arises from the gastric mucosal epithelium and severely affects patient health and quality of life. Luteolin (LUT) is a flavonoid found in vegetables and fruits with diverse functions. A large number of studies have confirmed that LUT has an antitumor effect. Therefore, this study is aimed at verifying whether LUT can exert antitumor effects in synergy with oxaliplatin (OXA). As such, we examined the effects of LUT, OXA, and their coadministration in a gastric adenocarcinoma cell line (SGC-7901). We used the MTT assay to quantify the proliferation of SGC-7901 cells, flow cytometry to detect the cell cycle and apoptosis, ELISA to detect the expression of cell-cycle-related proteins, and western blot to detect the expression of related apoptotic factors. The results of this study show that the combination of LUT and OXA inhibited SGC-7901 cell proliferation and induced apoptosis by altering cell-cycle proportions. In addition, the combination also activated Cyt c/caspase signaling in SGC-7901 cells. In summary, LUT synergy with OXA inhibited the proliferation of gastric cancer cells . The present study also elucidated the mechanism by which LUT potentiated the sensitivity of SGC-7901 cells to OXA through the Cyt c/caspase pathway.
. 胃癌是全球最常见的恶性肿瘤之一，起源于胃黏膜上皮，严重影响患者的健康和生活质量。木犀草素（LUT）是一种存在于蔬菜和水果中的类黄酮，具有多种功能。大量研究证实，LUT 具有抗肿瘤作用。因此，本研究旨在验证 LUT 是否可以与奥沙利铂（OXA）协同发挥抗肿瘤作用。为此，我们在胃腺癌细胞系（SGC-7901）中检查了 LUT、OXA 及其联合用药的作用。我们使用 MTT 测定法来量化 SGC-7901 细胞的增殖，使用流式细胞术检测细胞周期和细胞凋亡，使用 ELISA 检测细胞周期相关蛋白的表达，使用 Western blot 检测相关凋亡因子的表达。本研究结果表明，LUT 和 OXA 的联合使用通过改变细胞周期比例抑制了 SGC-7901 细胞的增殖并诱导了细胞凋亡。此外，联合用药还激活了 SGC-7901 细胞中的 Cyt c/caspase 信号通路。综上所述，LUT 与 OXA 的协同作用抑制了胃癌细胞的增殖。本研究还通过 Cyt c/caspase 通路阐明了 LUT 增强 SGC-7901 细胞对 OXA 敏感性的机制。
