State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang 315211, China.
Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315832, China.
Zool Res. 2020 Mar 18;41(2):123-137. doi: 10.24272/j.issn.2095-8137.2020.026.
Interleukin-34 (IL-34) is a novel cytokine that plays an important role in innate immunity and inflammatory processes by binding to the colony-stimulating factor-1 receptor (CSF-1R). However, information on the function of IL-34 in fish remains limited. In the present study, we identified an IL-34 homolog from mudskippers ( ). analysis showed that the mudskipper IL-34 (BpIL-34) was similar to other known IL-34 variants in sequence and structure and was most closely related to an orange-spotted grouper ( ) homolog. transcripts were constitutively expressed in various tissues, with the highest level of expression found in the brain. infection significantly up-regulated the mRNA expression of The recombinant mature BpIL-34 peptide (rBpIL-34) was purified and used to produce anti-rBpIL-34 IgG. Western blot analysis combined with PNGase F digestion revealed that native BpIL-34 in monocytes/macrophages (MOs/MФs) was N-glycosylated. , rBpIL-34 treatment enhanced the phagocytotic and bactericidal activity of mudskipper MOs/MФs, as well as the mRNA expression of pro-inflammatory cytokines like tumor necrosis factor α ( ) and in these cells. Furthermore, the knockdown of mudskipper ( ), but not mudskipper , significantly inhibited the rBpIL-34-mediated enhanced effect on MO/MФ function. In conclusion, our results indicate that mudskipper BpIL-34 modulates the functions of MOs/MФs via BpCSF-1R1.
白细胞介素 34（IL-34）是一种新型细胞因子，通过与集落刺激因子 1 受体（CSF-1R）结合，在先天免疫和炎症过程中发挥重要作用。然而，关于 IL-34 在鱼类中的功能信息仍然有限。在本研究中，我们从弹涂鱼中鉴定出一种 IL-34 同源物（）。序列和结构分析表明，弹涂鱼 IL-34（BpIL-34）与其他已知的 IL-34 变体在序列和结构上相似，与橙点石斑鱼（）同源物最为密切相关。在各种组织中均持续表达，在脑中表达水平最高。 感染显著上调了 的 mRNA 表达。重组成熟 BpIL-34 肽（rBpIL-34）被纯化并用于产生抗 rBpIL-34 IgG。Western blot 分析结合 PNGase F 消化表明，单核细胞/巨噬细胞（MOs/MФs）中的天然 BpIL-34 是 N-糖基化的。rBpIL-34 处理增强了弹涂鱼 MOs/MФs 的吞噬和杀菌活性，以及这些细胞中促炎细胞因子如肿瘤坏死因子 α（）和 的 mRNA 表达。此外，弹涂鱼（）的敲低，但不是弹涂鱼（）的敲低，显著抑制了 rBpIL-34 介导的对 MO/MФ 功能的增强作用。总之，我们的结果表明，弹涂鱼 BpIL-34 通过 BpCSF-1R1 调节 MOs/MФs 的功能。
