Jiang Yunyun, Chen Jiahao, Yen Kuangyu, Xu Jin
1 Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.
2 Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou, China.
Zebrafish. 2019 Apr;16(2):165-170. doi: 10.1089/zeb.2018.1685. Epub 2019 Feb 6.
Interleukin-34 (IL-34) is a known cytokine that plays an important role in the survival, proliferation, and differentiation of macrophages. In previous studies, IL-34 can induce macrophage migration through syndecan-1 or focal adhesion kinase and extracellular signal-related kinase 1 and 2 pathway. These studies mainly focused on in vitro experiments, but the effect of IL-34 on macrophage migration in vivo is less understood. In our study, we artificially induced macrophage, but not neutrophil, enrichment in the skin or liver by overexpressing IL-34 in epidermal cells or hepatocytes in zebrafish. Live imaging showed that the enrichment of macrophages in the liver is due to the direct attraction of macrophages by IL-34. Our results demonstrated that ectopically expressed IL-34 can induce macrophage migration to liver in vivo.
白细胞介素-34（IL-34）是一种已知的细胞因子，在巨噬细胞的存活、增殖和分化中发挥重要作用。在先前的研究中，IL-34可通过多配体蛋白聚糖-1或粘着斑激酶以及细胞外信号调节激酶1和2途径诱导巨噬细胞迁移。这些研究主要集中在体外实验，但IL-34对体内巨噬细胞迁移的影响了解较少。在我们的研究中，我们通过在斑马鱼的表皮细胞或肝细胞中过表达IL-34，人工诱导巨噬细胞而非中性粒细胞在皮肤或肝脏中富集。实时成像显示，肝脏中巨噬细胞的富集是由于IL-34对巨噬细胞的直接吸引。我们的结果表明，异位表达的IL-34可在体内诱导巨噬细胞向肝脏迁移。
