Laboratory of Reproductive Toxicology, Department of Medicine, State University of Central-West, Rua Simeao Camargo Varela de Sa, 03, 85040-080, Parana, Brazil.
Department of Physiology, Animal Endocrine and Reproductive Physiology Laboratory, Federal University of Paraná (UFPR), Centro Politécnico, 81531-980,PO Box 19031, Curitiba, Parana, Brazil.
Toxicology. 2020 Apr 30;436:152428. doi: 10.1016/j.tox.2020.152428. Epub 2020 Mar 6.
The increase in human infertility prevalence due to male reproductive disorders has been associated with extensive endocrine-disrupting chemical (EDC) exposure. Acrylamide (AA) is a compound formed spontaneously during heat processing of some foods that are mainly consumed by children and adolescents. In this study, we evaluated the prepubertal AA exposure effects on male adult reproductive physiology using a prepubertal experimental model to analyze the pubertal development, spermatogenesis hormones levels and genes expression involved in male reproductive function. This study is the first one to use the validated protocol to correlate the AA exposure with puberty development, as well as the AA-induced endocrine disrupting effects on reproductive axis. AA did not affect the age at puberty, the reproductive organ's weight and serum hormonal levels. AA reduces spermatogenesis, induces morphological and functional defects on sperm and alters transcript expression of sexual hormone receptors (Ar and Esr2), the transcript expression of Tnf, Egr2, Rhcg and Lrrc34. These findings suggest that excessive AA consumption may impair their reproductive capacity at adulthood, despite no changes in hormonal profile being observed.
由于男性生殖障碍导致的人类不孕不育患病率的增加与广泛的内分泌干扰化学物质（EDC）暴露有关。丙烯酰胺（AA）是一种在某些食物的热加工过程中自然形成的化合物，这些食物主要被儿童和青少年食用。在这项研究中，我们使用青春期前的实验模型评估了青春期前 AA 暴露对男性成年生殖生理学的影响，以分析涉及男性生殖功能的青春期发育、生精激素水平和基因表达。这是首次使用经过验证的方案将 AA 暴露与青春期发育相关联，以及 AA 对生殖轴的内分泌干扰作用。AA 不会影响青春期的年龄、生殖器官的重量和血清激素水平。AA 会降低精子发生，导致精子的形态和功能缺陷，并改变性荷尔蒙受体（Ar 和 Esr2）、Tnf、Egr2、Rhcg 和 Lrrc34 的转录表达。这些发现表明，尽管观察到激素谱没有变化，但过量的 AA 摄入可能会损害他们成年后的生殖能力。
