Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, University of Utrecht, Utrecht, the Netherlands.
Netherlands Proteomics Center, Utrecht, the Netherlands.
Nat Methods. 2020 Apr;17(4):395-398. doi: 10.1038/s41592-020-0770-7. Epub 2020 Mar 9.
We demonstrate single-particle charge detection mass spectrometry on an Orbitrap for the analysis of megadalton biomolecular assemblies. We establish that the signal amplitudes of individual ions scale linearly with their charge, which can be used to resolve mixed ion populations, determine charge states and thus also determine the masses of individual ions. This enables the ultrasensitive analysis of heterogeneous protein assemblies including immunoglobulin oligomers, ribosomes, proteinaceous nanocontainers and genome-packed adeno-associated viruses.
我们在轨道阱上展示了用于分析兆道尔顿生物大分子组装体的单颗粒电荷检测质谱法。我们证实了单个离子的信号幅度与其电荷呈线性比例关系，这可用于解析混合离子群体，确定电荷状态，从而确定单个离子的质量。这使得对包括免疫球蛋白寡聚体、核糖体、蛋白质纳米容器和基因组包装的腺相关病毒在内的异质蛋白质组装体的超灵敏分析成为可能。
