Park Jung Uoon, Cho Jin Sook, Kim Jong Seok, Kim Hyun Kyu, Jo Young Hee, Rahman Md Aziz Abdur, Lee Young Ik
Industrial Bioresource Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, South Korea.
Lee's Biotech Co., KRIBB, Daejeon, South Korea.
Front Pharmacol. 2020 Feb 20;11:4. doi: 10.3389/fphar.2020.00004. eCollection 2020.
is one of the most widespread infections involved in the pathogenesis of chronic gastritis, peptic ulcer, and gastric cancer. Hence, there is an urgent need to develop medications against . This study aimed to evaluate synergistic effect of (RF) and Hance (UL) against . Antibacterial susceptibility of each extract either separately or in combination was studied against two standard strains and 11 clinical isolates using agar dilution method. The effect of the extracts on inoculated Balb/c mice model was also studied using single dosing (100 mg/kg each) approach. The MIC of RF and UL were more than 100 and 200 µg/ml, respectively, against the tested strains. However, simultaneous treatment with RF and UL at 75 and 50 µg/ml, respectively, showed decreased viable cell number, MIC, and at 75 µg/ml each showed synergic effect with MIC. On inoculated Balb/c mice model, RF and UL separately (100 mg/kg each) showed moderate anti- effect, while simultaneous treatment of RF and UL with same dose showed significant synergistic anti-gastric effects in stomach. The results showed a significant synergistic effect of plants extract against infection and eventually gastric mucosal damage. Our finding could be considered a valuable support in the treatment of induced gastritis and may contribute to the development of new and safe combined herbal product as anti- regimens.
是参与慢性胃炎、消化性溃疡和胃癌发病机制的最广泛传播的感染之一。因此，迫切需要开发针对它的药物。本研究旨在评估（某种提取物，原文未明确写出全称，暂用RF代替）和（某种提取物，原文未明确写出全称，暂用UL代替）对它的协同作用。采用琼脂稀释法研究了每种提取物单独或联合使用时对两种标准菌株和11株临床分离株的抗菌敏感性。还采用单次给药（各100mg/kg）方法研究了提取物对接种了它的Balb/c小鼠模型的影响。RF和UL对受试菌株的最低抑菌浓度分别大于100和200μg/ml。然而，分别以75和50μg/ml的浓度同时使用RF和UL时，活菌数减少，最低抑菌浓度降低，且各以75μg/ml使用时显示出与最低抑菌浓度的协同作用。在接种了它的Balb/c小鼠模型上，RF和UL单独使用（各100mg/kg）显示出中等程度的抗它作用，而相同剂量的RF和UL同时使用在胃中显示出显著的协同抗胃作用。结果表明植物提取物对它的感染以及最终的胃黏膜损伤具有显著的协同作用。我们的发现可为治疗它引起的胃炎提供有价值的支持，并可能有助于开发新的、安全的联合草药产品作为抗它方案。
