Chedid Elsa, Rizou Myrto, Kalaitzis Panagiotis
Department of Horticultural Genetics & Biotechnology, Mediterranean Agronomic Institute of Chania, Crete, Greece.
Food Chem X. 2020 Feb 24;6:100082. doi: 10.1016/j.fochx.2020.100082. eCollection 2020 Jun 30.
A plethora of biotechnological methodologies is used to authenticate quality olive oils. Among the DNA-based approaches, SNPs and SSRs combined with high resolution melting (HRM) provide certain advantages such as speed, simplicity and reliability. SNP-HRM and SSR-HRM were used for the authentication of monovarietal olive oils as well as the quantification of varietal composition in olive oil DNA admixtures and olive oil blends of two different cultivars. The SSR-HRM was more efficient in distinguishing monovarietal olive oils while the SNP-HRM assay was more reliable in discriminating olive oil blends. HRM was also used for the detection of adulteration of olive oil with oils of different plant origin by using plastid indels and SNPs. The trnL-indels-HRM showed higher discrimination power than the -SNP-HRM in determining adulteration in olive oil. These results indicate that traceability of adulteration might be more reliable than authentication of the varietal origin in olive oil blends.
大量生物技术方法被用于鉴定优质橄榄油。在基于DNA的方法中，单核苷酸多态性（SNPs）和简单序列重复（SSRs）结合高分辨率熔解曲线分析（HRM）具有速度快、操作简单和可靠性高等优势。单核苷酸多态性-高分辨率熔解曲线分析（SNP-HRM）和简单序列重复-高分辨率熔解曲线分析（SSR-HRM）被用于单一品种橄榄油的鉴定，以及橄榄油DNA混合物和两种不同品种橄榄油混合油中品种成分的定量分析。SSR-HRM在区分单一品种橄榄油方面更有效，而SNP-HRM分析在鉴别橄榄油混合油方面更可靠。HRM还通过使用质体插入缺失和单核苷酸多态性来检测橄榄油与不同植物来源油脂的掺假情况。在确定橄榄油掺假方面，trnL插入缺失-高分辨率熔解曲线分析（trnL-indels-HRM）比单核苷酸多态性-高分辨率熔解曲线分析（-SNP-HRM）具有更高的鉴别能力。这些结果表明，在橄榄油混合油中，掺假的可追溯性可能比品种来源的鉴定更可靠。
