Lian Dong-Sheng, Zeng Hua-Song
Guangzhou Women and Children's Medical Center of Guangzhou Medical University, NO. 9 at Jinsui Rd., Tianhe District, Guangzhou, Guangdong, China.
Compr Rev Food Sci Food Saf. 2017 Nov;16(6):1281-1295. doi: 10.1111/1541-4337.12297. Epub 2017 Sep 12.
Food safety and food production are closely related to the health of consumers. Food-related accidents often cause tremendous losses of personnel and property. Thus, rapid detection and analysis of ingredients in food, tracing food sources, studying the optimal conditions for food production, and more are vital for preventing incidents related to safety. Conventional analysis based on proteomics, microbial cultures, and morphology, as well as biochemical tests based on metabonomics, are considered gold standards and used frequently, but they are labor-intensive, time-consuming, tedious, error-prone, and incapable of meeting the demand for rapid and precise detection at a large scale. Alternative detection methods that utilize capillary electrophoresis have the advantages of high efficiency, high throughput, high speed, and automation; these methods are coupled with various nucleic acid detection strategies to overcome the drawbacks of traditional identification methods, and to prevent false results. Therefore, this review focuses on the application of capillary electrophoresis based on nucleic acid detection in food analysis and provides an introduction to the limitations, advantages, and future developments of this approach.
食品安全与食品生产与消费者健康密切相关。与食品相关的事故往往会造成巨大的人员和财产损失。因此，快速检测和分析食品中的成分、追溯食品来源、研究食品生产的最佳条件等对于预防安全事故至关重要。基于蛋白质组学、微生物培养和形态学的传统分析方法，以及基于代谢组学的生化测试方法，被视为金标准且经常使用，但它们劳动强度大、耗时、繁琐、容易出错，无法满足大规模快速精确检测的需求。利用毛细管电泳的替代检测方法具有高效、高通量、高速和自动化的优点；这些方法与各种核酸检测策略相结合，以克服传统鉴定方法的缺点，并防止出现错误结果。因此，本综述重点介绍基于核酸检测的毛细管电泳在食品分析中的应用，并介绍该方法的局限性、优点和未来发展。
