School of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Patia, Bhubaneswar, Odisha 751024, India.
Food Funct. 2020 Mar 1;11(3):2555-2572. doi: 10.1039/c9fo02249j. Epub 2020 Mar 10.
The consumption of probiotic based foods due to their health promoting effects has increased over the years. The consumption of probiotic based foods, due to their health promoting effects, has increased over the years. Due to this rising demands, the application platforms of the food industry have directed their efforts in the development of novel functional probiotic beverages. The presence of dietary phenolics and other beneficial components has made fruit-based matrices more popular for probiotic delivery. However, very few studies to date have evaluated the impact of carrier matrices on the in vivo functionality of probiotic strains with specific biomarkers and targeted towards specific disease conditions. In this study, we investigated the effect of a sea buckthorn (Hippophae rhamnoides L.) based, malt supplemented, beverage matrix (SBT + M) on the physiological characteristics of the probiotic strain L. rhamnosus GG (ATCC 53103) (LR). No significant difference in the auto-aggregation and co-aggregation rates of LR fortified in SBT + M was observed compared to LR alone. Storage conditions (4 °C for 14 days) did not affect the survival and the adhesion potential of LR to the HCT116 cell line. More importantly, this study also determined the protective effect of SBT + M on the in vivo functionality of LR in alleviating LPS induced inflammation in zebrafish (Danio rerio). The results showed that administration with SBT + M + LR significantly alleviated the LPS induced colonic damage, evidenced by the decrease in the overall histological and goblet cell scores compared to the LPS-treated group. The protective effect of SBT + M + LR was higher compared to the effects of SBT + M or LR alone. The colonic expression of pro-inflammatory cytokines (TNF-α and IL-1β) showed a marked decline by 84.7% and 86.5% respectively. The highest expression of the anti-inflammatory cytokine IL-10 was recorded in the SBT + M + LR treated group (p < 0.001). The overall results reveal that the administration of SBT + M + LR showed the best effects in alleviating the LPS induced intestinal damage compared to the other test groups (SBT + M and LR). These protective effects could be attributed to the phenolic compounds present in the SBT matrix. Our study indicates the contributory role of the carrier matrix and merits further investigation for its use as a potential therapeutic agent for gastrointestinal inflammation.
由于其促进健康的作用，益生菌食品的消费多年来一直在增加。由于这种需求的增长，食品行业的应用平台已经将其努力方向转向开发新型功能性益生菌饮料。由于膳食酚类化合物和其他有益成分的存在，基于水果的基质更受欢迎，可用于益生菌输送。然而，迄今为止，很少有研究评估载体基质对具有特定生物标志物和针对特定疾病条件的益生菌菌株在体内功能的影响。在这项研究中，我们研究了沙棘（Hippophae rhamnoides L.）为基础，添加麦芽的饮料基质（SBT + M）对益生菌菌株 L. rhamnosus GG（ATCC 53103）（LR）生理特性的影响。与单独的 LR 相比，在 SBT + M 中强化的 LR 的自动聚集和共聚率没有显著差异。储存条件（4°C 14 天）不会影响 LR 的存活率和对 HCT116 细胞系的粘附能力。更重要的是，这项研究还确定了 SBT + M 对 LR 在减轻斑马鱼（Danio rerio）中 LPS 诱导的炎症的体内功能的保护作用。结果表明，与 LPS 处理组相比，用 SBT + M + LR 给药可显著减轻 LPS 诱导的结肠损伤，这表现在整体组织学和杯状细胞评分的降低。SBT + M + LR 的保护作用高于 SBT + M 或 LR 单独的作用。结肠中促炎细胞因子（TNF-α和 IL-1β）的表达分别下降了 84.7%和 86.5%。SBT + M + LR 处理组中抗炎细胞因子 IL-10 的表达最高（p <0.001）。总体结果表明，与其他测试组（SBT + M 和 LR）相比，SBT + M + LR 给药在减轻 LPS 诱导的肠道损伤方面效果最佳。这些保护作用可能归因于 SBT 基质中存在的酚类化合物。我们的研究表明载体基质的贡献作用，并值得进一步研究其作为治疗胃肠道炎症的潜在治疗剂的用途。
