School of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Patia, Bhubaneswar, Odisha 751024. India.
School of Biotechnology, Kalinga Institute of Industrial Technology, Deemed to be University, Patia, Bhubaneswar, Odisha 751024. India.
Food Res Int. 2021 Nov;149:110661. doi: 10.1016/j.foodres.2021.110661. Epub 2021 Aug 25.
Different lines of evidences from clinical, epidemiological and biochemical studies have established that optimal nutrition including probiotic and fruit phenolics can mitigate the risk and morbidity associated with some chronic diseases. The basis for this observation is the potential synergies that may exist between probiotic strains and different bioactive components of food matrices. This study was conceptualized to compare the efficiency of a probiotic strain in two different fruit matrices. Two fruits, viz., sea buckthorn (Hippophae rhamnoides) (SBT) and apples (Malus pumila) (APJ) were chosen and the anti-inflammatory effects of L. rhamnosus GG (ATCC 53103) (LR) fortified in SBT and APJ were analysed against dextran sulphate sodium (DSS) induced colitis in zebrafish (Danio rerio). The results showed that administration of probiotic (LR) fortified, malt supplemented SBT beverage (SBT + M + LR) had better restorative potential on the intestinal barrier function and mucosal damage, in comparison to LR fortified, malt supplemented APJ beverage (APJ + M + LR). SBT + M + LR demonstrated adequate anti-oxidant potential by enhancing the CAT, SOD, GPx and GSH activities, impaired due to DSS administration. The increase in the expressions of toll like receptor (TLR)-2, TLR-4 and TLR-5 induced by DSS were significantly inhibited by SBT + M + LR administration. Gene expression of pro-inflammatory markers, (NF-κB, TNF-α, IL-1β, IL-6, IL-8, CCL20, MPO and MMP9) were attenuated by SBT + M + LR treatment in intestinal tissues of DSS-treated zebrafishes. Notably, SBT + M + LR increased the expression of anti-inflammatory cytokine, IL-10. The study provides evidence that specific interactions between fruit matrix and probiotic strain can provide adjunct therapeutic strategy to manage intestinal inflammation.
来自临床、流行病学和生化研究的不同证据表明，最佳营养包括益生菌和水果类黄酮可以降低某些慢性疾病的风险和发病率。这一观察结果的基础是益生菌菌株和不同食物基质生物活性成分之间可能存在的协同作用。本研究旨在比较益生菌菌株在两种不同水果基质中的效率。选择了两种水果，即沙棘（Hippophae rhamnoides）（SBT）和苹果（Malus pumila）（APJ），并分析了在富含益生菌的 SBT 和 APJ 中添加嗜酸乳杆菌 GG（ATCC 53103）（LR）对斑马鱼（Danio rerio）葡聚糖硫酸钠（DSS）诱导的结肠炎的抗炎作用。结果表明，与富含益生菌的、添加麦芽糖的 APJ 饮料（APJ+M+LR）相比，富含益生菌的、添加麦芽糖的 SBT 饮料（SBT+M+LR）对肠道屏障功能和粘膜损伤有更好的修复作用。SBT+M+LR 表现出足够的抗氧化潜力，通过增强 CAT、SOD、GPx 和 GSH 的活性来增强 CAT、SOD、GPx 和 GSH 的活性，而这些活性因 DSS 的给药而受损。DSS 给药引起的 TLR-2、TLR-4 和 TLR-5 的表达增加被 SBT+M+LR 给药显著抑制。SBT+M+LR 处理可减轻肠道组织中促炎标志物（NF-κB、TNF-α、IL-1β、IL-6、IL-8、CCL20、MPO 和 MMP9）的基因表达。值得注意的是，SBT+M+LR 增加了抗炎细胞因子 IL-10 的表达。该研究提供的证据表明，水果基质和益生菌菌株之间的特定相互作用可以提供辅助治疗策略来管理肠道炎症。
