Zhi Kexin, Gong Fanwen, Chen Lele, Li Zezheng, Li Xiang, Mei Huadi, Fu Chenxing, Zhao Yurong, Liu Zhuying, He Jianhua
College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
College of Animal Science and Technology, Hunan Biological and Electromechanical Polytechnic, Changsha 410128, China.
Animals (Basel). 2024 Jul 15;14(14):2073. doi: 10.3390/ani14142073.
The experiment investigated the effects of sea-buckthorn flavonoids (SF) on lipopolysaccharide (LPS)-challenged broilers. A total of 288 one-day-old male broilers were randomly assigned to 4 groups, with 6 replicates of 12 broilers each. The experiment lasted for 20 days. The diet included two levels of SF (0 or 1000 mg/kg) and broilers intraperitoneally injected with 500 μg/kg LPS on 16, 18, and 20 days, or an equal amount of saline. LPS challenge decreased final body weight, average daily gain, and average daily feed intake, increased feed-to-gain ratio, and elevated serum IL-1β, IL-2, TNF-α, D-LA, and endotoxin levels. Moreover, it resulted in a reduction in the IL-10 level. LPS impaired the intestinal morphology of the duodenum, jejunum, and ileum, down-regulated the mRNA relative expression of Occludin, ZO-1, and MUC-2 in the jejunum mucosa, up-regulated the mRNA relative expression of TLR4, MyD88, NF-κB, and IL-1β, and increased the relative abundance of in broilers ( < 0.05). However, SF supplementation mitigated the decrease in growth performance, reduced serum IL-1β, IL-2, and D-LA levels, increased IL-10 levels, alleviated intestinal morphological damage, up-regulated mRNA expression of Occludin and ZO-1, down-regulated the mRNA expression of TLR4, NF-κB, and IL-lβ in jejunum mucosal ( < 0.05), and SF supplementation presented a tendency to decrease the relative abundance of proteobacteria (0.05 < < 0.1). Collectively, incorporating SF can enhance the growth performance, alleviate serum inflammation, and improve the intestinal health of broilers, effectively mitigating the damage triggered by LPS-challenges.
本实验研究了沙棘黄酮（SF）对脂多糖（LPS）攻击的肉鸡的影响。总共288只1日龄雄性肉鸡被随机分为4组，每组6个重复，每个重复12只肉鸡。实验持续20天。日粮包含两个水平的SF（0或1000毫克/千克），肉鸡在第16、18和20天腹腔注射500微克/千克LPS，或等量的生理盐水。LPS攻击降低了末体重、平均日增重和平均日采食量，提高了料重比，并升高了血清IL-1β、IL-2、TNF-α、D-LA和内毒素水平。此外，它还导致IL-10水平降低。LPS损害了十二指肠、空肠和回肠的肠道形态，下调了空肠黏膜中Occludin、ZO-1和MUC-2的mRNA相对表达，上调了TLR4、MyD88、NF-κB和IL-1β的mRNA相对表达，并增加了肉鸡中（<0.05）的相对丰度。然而，补充SF减轻了生长性能的下降，降低了血清IL-1β、IL-2和D-LA水平，提高了IL-10水平，减轻了肠道形态损伤，上调了Occludin和ZO-1的mRNA表达，下调了空肠黏膜中TLR4、NF-κB和IL-1β的mRNA表达（<0.05），并且补充SF呈现出降低变形菌门相对丰度的趋势（0.05<<0.1）。总体而言，添加SF可以提高肉鸡的生长性能，减轻血清炎症，并改善肠道健康，有效减轻LPS攻击引发的损伤。
