Ma Jun, Lin Weifeng, Xu Liangbo, Liu Sihang, Xue Weili, Chen Shengfu
State Key Laboratory of Chemical Engineering, Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
Institute of Zhejiang University, Quzhou 324000, China.
Langmuir. 2020 Mar 31;36(12):3251-3259. doi: 10.1021/acs.langmuir.0c00006. Epub 2020 Mar 19.
Long-term resistance of biomaterials to the bacterial biofilm formation without antibiotic or biocide is highly demanded for biomedical applications. In this work, a novel biodegradable biomaterial with excellent capability to prevent long-term bacterial biofilm formation is prepared by the following two steps. Ethylcarboxybetaine ester analogue methacrylate (ECBEMA), poly(ethylene glycol) monomethacrylate (PEGMA), and 3-methacryloxypropyletris(trimethylsiloxy)silane (TRIS) were copolymerized to obtain p(ECBEMA-PEGMA-TRIS) (PEPT). Then, PEPT was cross-linked by isocyanate-terminated polylactic acid (IPDI-PLA-IPDI) to obtain the final PEPT-PLA ( and are the number-average molecular weights () of PEPT and PLA, respectively) with optimal mechanical strength and adjustable surface regeneration rate. Static contact angle measurement, protein adsorption measurement, and attenuated total reflectance infrared (ATR-IR) results show that the PEPT19800-PLA800 film surface can generate a zwitterionic layer to resist nonspecific protein adsorption after surface hydrolysis. Quartz crystal microbalance with dissipation (QCM-D) results indicates that the PEPT19800-PLA800 film can undergo gradual degradation of the surface layer at the lowest swelling rate. Particularly, this material can efficiently resist the bacterial biofilm formation of both Gram-positive bacteria and Gram-negative bacteria over 14 and 6 days, respectively. Moreover, the material also shows an ideal self-healing feature to adapt to harsh conditions. Thus, this nonfouling material shows great potential in biomedical applications and marine antifouling coatings without antibiotic or biocide.
生物医学应用对生物材料在无抗生素或杀菌剂情况下长期抵抗细菌生物膜形成的能力有很高要求。在这项工作中，通过以下两个步骤制备了一种具有优异长期防止细菌生物膜形成能力的新型可生物降解生物材料。将乙基羧基甜菜碱酯类似物甲基丙烯酸酯（ECBEMA）、聚乙二醇单甲基丙烯酸酯（PEGMA）和3-甲基丙烯酰氧基丙基三（三甲基硅氧基）硅烷（TRIS）共聚以获得p（ECBEMA-PEGMA-TRIS）（PEPT）。然后，PEPT通过异氰酸酯封端的聚乳酸（IPDI-PLA-IPDI）交联，以获得具有最佳机械强度和可调节表面再生速率的最终PEPT-PLA（ 和 分别是PEPT和PLA的数均分子量（ ））。静态接触角测量、蛋白质吸附测量和衰减全反射红外（ATR-IR）结果表明，PEPT19800-PLA800薄膜表面在表面水解后可产生两性离子层以抵抗非特异性蛋白质吸附。石英晶体微天平耗散（QCM-D）结果表明，PEPT19800-PLA800薄膜可以以最低的溶胀率进行表面层的逐渐降解。特别地，这种材料可以分别在14天和6天内有效抵抗革兰氏阳性菌和革兰氏阴性菌的细菌生物膜形成。此外，该材料还表现出理想的自愈特性以适应恶劣条件。因此，这种防污材料在无抗生素或杀菌剂的生物医学应用和海洋防污涂料中显示出巨大潜力。
