Faculty of Materials Science and Engineering , South China University of Technology , Guangzhou 510640 , P. R. China.
ACS Appl Mater Interfaces. 2018 Apr 4;10(13):11213-11220. doi: 10.1021/acsami.8b00962. Epub 2018 Mar 21.
Persistent protein resistance is critical for marine antibiofouling. We have prepared copolymer of 2-methylene-1,3-dioxepane (MDO), tertiary carboxybetaine ester (TCB), and 7-methacryloyloxy-4-methylcoumarin (MAMC) via radical ring-opening polymerization, where MDO, TCB, and MAMC make the polymer degradable, protein resistible, and photo-cross-linkable, respectively. Our study shows that the polymer can well adhere to the substrate with controlled degradation and water adsorption rate in artificial seawater (ASW). Particularly, the polymer film can generate zwitterions via surface hydrolysis in ASW. Quartz crystal microbalance with dissipation measurements reveal that such hydrolysis-induced zwitterionic surface can effectively resist nonspecific protein adsorption. Moreover, the surface can inhibit the adhesion of marine bacteria Pseudomonas sp. and Vibrio alginolyticus as well as clinical bacterium Escherichia coli.
持久的蛋白质抗性对于海洋抗菌生物污损至关重要。我们通过自由基开环聚合制备了 2-亚甲基-1,3-二氧戊环（MDO）、叔羧基甜菜碱酯（TCB）和 7-丙烯酰氧基-4-甲基香豆素（MAMC）的共聚物，其中 MDO、TCB 和 MAMC 分别使聚合物具有可降解性、抗蛋白质性和光交联性。我们的研究表明，该聚合物可以在人工海水中（ASW）很好地附着在基底上，并具有可控的降解和水吸附率。特别是，聚合物薄膜可以在 ASW 中通过表面水解生成两性离子。石英晶体微天平耗散测量表明，这种水解诱导的两性离子表面可以有效地抵抗非特异性蛋白质吸附。此外，该表面还可以抑制海洋细菌假单胞菌和弧菌以及临床细菌大肠杆菌的粘附。
