Department of Metallurgical and Materials Engineering, Middle East Technical University, Çankaya, Ankara, Turkey.
Institute of Biomedical Engineering, Boğaziçi University, Istanbul, Turkey.
J Biomed Mater Res B Appl Biomater. 2020 Oct;108(7):2743-2753. doi: 10.1002/jbm.b.34604. Epub 2020 Mar 10.
Tantalum possesses remarkable chemical and mechanical properties, and thus it is considered to be one of the next generation implant materials. However, the biological properties of tantalum remain to be improved for its use in tissue engineering applications. To enhance its cellular interactions, implants made of tantalum could be modified to obtain nanofeatured surfaces via the electrochemical anodization process. In this study, anodization parameters were adjusted to obtain a nanoporous surface morphology on tantalum surfaces and systematically altered to control the pore sizes from 25 to 65 nm using an aqueous HF:H SO electrolyte. Results indicated the formation of Ta O -based nanoporous surface layers, which had up to 28% more surface area and increased nanophase roughness (more than twofolds) compared to nonporous tantalum upon the anodization. It was observed that the nanoporous tantalum oxide surfaces promoted nearly 25% more fibroblast proliferation at 5 days in vitro and 15.5% more cellular spreading. Thus, nanoporous tantalum oxide surfaces can be used to increase biological interactions of the cells and provide a means of improving bioactivity of tantalum for biomaterial applications.
钽具有显著的化学和机械性能，因此被认为是下一代植入材料之一。然而，为了将其应用于组织工程，钽的生物性能仍需改善。为了增强其细胞相互作用，可以通过电化学阳极氧化工艺对钽制成的植入物进行修饰，以获得具有纳米结构的表面。在这项研究中，通过调整阳极氧化参数，使用含有 HF 和 H2SO4 的水溶液在钽表面上获得了纳米多孔形貌，并系统地改变参数来控制孔径大小，从 25nm 到 65nm。结果表明，形成了基于 Ta2O5 的纳米多孔表面层，与非多孔钽相比，其表面积增加了 28%，纳米相粗糙度增加了两倍多（超过两倍）。观察到纳米多孔氧化钽表面在体外培养 5 天时促进了近 25%的成纤维细胞增殖，细胞铺展增加了 15.5%。因此，纳米多孔氧化钽表面可用于增加细胞的生物相互作用，并为生物材料应用提供提高钽生物活性的方法。
