Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, Department of Pharmaceutical Analysis, Fujian Medical University, Fuzhou 350004, China.
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
ACS Appl Mater Interfaces. 2020 Apr 1;12(13):14876-14883. doi: 10.1021/acsami.0c00778. Epub 2020 Mar 19.
Protein-supported nanoparticles have a great significance in scientific and nanotechnology research because of their "green" process, low cost-in-use, good biocompatibility, and some interesting properties. Ruthenium oxide nanoparticles (RuONPs) have been considered to be an important member in nanotechnology research. However, the biosynthetic approach of RuONPs is relatively few compared to those of other nanoparticles. To address this challenge, this work presented a new way for RuONP synthesis (BSA-RuONPs) supported by bovine serum albumin (BSA). BSA-RuONPs are confirmed to exert peroxidase-like activity, electrocatalytic activity, in vitro salt resistance (2 M NaCl), and biocompatibility. Results indicate that BSA-RuONPs have higher affinity binding for 3,3',5,5'-tetramethylbenzidine or HO than bare RuONPs. Moreover, BSA turns out to be a crucial factor in promoting the stability of RuONPs. Taking the advantages of these improved properties, we established colorimetric (linear range from 2 to 800 μM, a limit of detection of 1.8 μM) and electrochemical (linear range from 0.4 to 3850 μM, a limit of detection of 0.18 μM) biosensors for monitoring in situ HO secretion from living MCF-7 cells. Herein, this work offers a new biosynthesis strategy to obtain BSA-RuONPs and sheds light on the sensitive biosensors to monitor the HO secreted from living cells for promising applications in the fields of nanotechnology, biology, biosensors, and medicine.
蛋白质支持的纳米粒子因其“绿色”过程、低成本、良好的生物相容性和一些有趣的特性，在科学和纳米技术研究中具有重要意义。氧化钌纳米粒子（RuONPs）被认为是纳米技术研究的重要成员。然而，与其他纳米粒子相比，RuONPs 的生物合成方法相对较少。为了解决这一挑战，本工作提出了一种新的 RuONP 合成方法（BSA-RuONPs），由牛血清白蛋白（BSA）支持。BSA-RuONPs 被证实具有过氧化物酶样活性、电催化活性、体外耐盐性（2 M NaCl）和生物相容性。结果表明，BSA-RuONPs 对 3,3',5,5'-四甲基联苯胺或 HO 的亲和力高于裸 RuONPs。此外，BSA 被证明是促进 RuONPs 稳定性的关键因素。利用这些改善的特性，我们建立了比色（线性范围从 2 到 800 μM，检测限为 1.8 μM）和电化学（线性范围从 0.4 到 3850 μM，检测限为 0.18 μM）生物传感器，用于原位监测活 MCF-7 细胞中 HO 的分泌。本文提供了一种新的生物合成策略来获得 BSA-RuONPs，并为监测活细胞分泌的 HO 的敏感生物传感器提供了启示，有望在纳米技术、生物学、生物传感器和医学等领域得到应用。
