National Collection of Industrial Microorganisms (NCIM), CSIR-National Chemical Laboratory (NCL), Pune, India.
National Collection of Industrial Microorganisms (NCIM), CSIR-National Chemical Laboratory (NCL), Pune, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Ecotoxicol Environ Saf. 2020 May;194:110443. doi: 10.1016/j.ecoenv.2020.110443. Epub 2020 Mar 7.
Anthropogenic activities mediated antibiotic resistance genes (ARGs) in the pristine aquatic bodies (lakes) is raising concern worldwide. Long read shotgun sequencing was used to assess taxonomic diversity, distribution of ARGs and metal resistance genes (MRGs) and mobile genetic elements (MGEs) in six sites within hypersaline Lonar soda lake (India) prone to various anthropogenic activities. Proteobacteria and Euryarchaeota were dominant phyla under domain Bacteria and Archaea respectively. Higher abundance of Bacteroidetes was pragmatic at sites 18LN5 and 18LN6. Functional analysis indicated 26 broad-spectrum ARGs types, not reported earlier in this ecosystem. Abundant ARG types identified were multidrug efflux, glycopepetide, bacitracin, tetracycline and aminogylcoside resistance. Sites 18LN1 and 18LN5 depicted 167 and 160 different ARGs subtypes respectively and rpoB2, bcrA, tetA(48), mupA, ompR, patA, vanR and multidrug ABC transporter genes were present in all samples. The rpoB2 gene was dominant in 18LN1, whereas bcrA gene in 18LN2-18LN6 sites. Around 24 MRGs types were detected with higher abundance of arsenic in 18LN1 and copper in 18LN2-18LN6, signifying metal contamination linked to MRGs. The bacterial taxa Pseudomonas, Thioalkalivibrio, Burkholderia, Clostridium, Paenibacillus, Bacillus and Streptomyces were significantly associated with ARGs. This study highlights the resistomic hotspots in the lake for deploying policies for conservation efforts.
人为活动介导的抗生素抗性基因（ARGs）在原始水体（湖泊）中引起了全球关注。本研究采用长读 shotgun 测序技术，评估了印度盐度极高的 Lonar 苏打湖中六个位点的分类多样性、ARGs 和金属抗性基因（MRGs）及移动遗传元件（MGEs）的分布。在细菌域和古菌域中，变形菌门和广古菌门分别为主要的门。在 18LN5 和 18LN6 位点，拟杆菌门的丰度较高。功能分析表明，在该生态系统中，以前没有报道过 26 种广谱 ARG 类型。鉴定出的丰富的 ARG 类型包括多药外排、糖肽、杆菌肽、四环素和氨基糖苷抗性。18LN1 和 18LN5 位点分别显示了 167 和 160 种不同的 ARG 亚型，所有样品中均存在 rpoB2、bcrA、tetA(48)、mupA、ompR、patA、vanR 和多药 ABC 转运蛋白基因。rpoB2 基因在 18LN1 中占优势，而 bcrA 基因在 18LN2-18LN6 位点占优势。检测到约 24 种 MRGs 类型，18LN1 中砷含量较高，18LN2-18LN6 中铜含量较高，表明与 MRGs 相关的金属污染。假单胞菌、硫辛酸杆菌、伯克霍尔德菌、梭菌、类芽孢杆菌、芽孢杆菌和链霉菌等细菌类群与 ARGs 显著相关。本研究突出了湖泊中的抗性热点，为制定保护政策提供了依据。
