Institute of Nuclear Physics Polish Academy of Sciences, PL-31-342 Krakow, Poland.
Department of Clinical Immunology, Institute of Pediatrics, Jagiellonian University Medical College, PL-30-663 Krakow, Poland.
Int J Mol Sci. 2020 Mar 6;21(5):1826. doi: 10.3390/ijms21051826.
Colon cancer constitutes 33% of all cancer cases in humans and the majority of patients with metastatic colon cancer still have poor prognosis. An important role in cancer development is the communication between cancer and normal cells. This may occur, among others, through extracellular vesicles (including microvesicles) (MVs), which are being released by both types of cells. MVs may regulate a diverse range of biological processes and are considered as useful cancer biomarkers. Herein, we show that similarity in the general chemical composition between colon cancer cells and their corresponding tumor-derived microvesicles (TMVs) does exist. These results have been confirmed by spectroscopic methods for four colon cancer cell lines: HCT116, LoVo, SW480, and SW620 differing in their aggressiveness/metastatic potential. Our results show that Raman and Fourier Transform InfraRed (FTIR) analysis of the cell lines and their corresponding TMVs did not differ significantly in the characterization of their chemical composition. However, hierarchical cluster analysis of the data obtained by both of the methods revealed that only Raman spectroscopy provides results that are in line with the molecular classification of colon cancer, thus having potential clinical relevance.
结肠癌占人类所有癌症病例的 33%，大多数转移性结肠癌患者的预后仍然较差。癌症发展中的一个重要角色是癌症和正常细胞之间的通讯。这种通讯可能发生在细胞外囊泡（包括微泡）（MVs）中，这些囊泡由这两种类型的细胞释放。MVs 可以调节多种不同的生物过程，并被认为是有用的癌症生物标志物。在此，我们证明了结肠癌细胞与其相应的肿瘤衍生的微泡（TMVs）之间在一般化学成分上存在相似性。这一结果已经通过光谱方法得到了四种结肠癌细胞系（HCT116、LoVo、SW480 和 SW620）的证实，这些细胞系在侵袭性/转移性潜力方面存在差异。我们的结果表明，对细胞系及其相应 TMVs 的拉曼和傅里叶变换红外（FTIR）分析在其化学成分的表征上没有显著差异。然而，对两种方法获得的数据进行层次聚类分析表明，只有拉曼光谱提供的结果与结肠癌的分子分类一致，因此具有潜在的临床相关性。
