Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, Japan.
Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.
Int J Mol Sci. 2020 Mar 6;21(5):1829. doi: 10.3390/ijms21051829.
Solution NMR spectroscopy is a unique and powerful technique that has the ability to directly connect the structural dynamics of proteins in physiological conditions to their activity and function. Here, we summarize recent studies in which solution NMR contributed to the discovery of relationships between key dynamic properties of proteins and functional mechanisms in important biological systems. The capacity of NMR to quantify the dynamics of proteins over a range of time scales and to detect lowly populated protein conformations plays a critical role in its power to unveil functional protein dynamics. This analysis of dynamics is not only important for the understanding of biological function, but also in the design of specific ligands for pharmacologically important proteins. Thus, the dynamic view of structure provided by NMR is of importance in both basic and applied biology.
溶液核磁共振波谱学是一种独特而强大的技术，它能够将生理条件下蛋白质的结构动力学与其活性和功能直接联系起来。在这里，我们总结了最近的一些研究，这些研究表明溶液核磁共振波谱学有助于发现蛋白质关键动力学特性与重要生物系统中功能机制之间的关系。NMR 能够定量检测蛋白质在一系列时间尺度上的动力学，并检测低丰度蛋白质构象，这对于揭示功能蛋白质动力学至关重要。这种动力学分析不仅对于理解生物学功能很重要，而且对于药理学上重要的蛋白质的特定配体的设计也很重要。因此，NMR 提供的结构动态视图在基础和应用生物学中都很重要。
