溶液核磁共振揭示多跨螺旋膜蛋白中与功能相关的动力学

Takeuchi Koh, Kofuku Yutaka, Imai Shunsuke, Ueda Takumi, Tokunaga Yuji, Toyama Yuki, Shiraishi Yutaro, Shimada Ichio

Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Aomi, Koto, Tokyo 135-0064, Japan.

Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Bunkyo, Tokyo 113-0033, Japan.

Membranes (Basel). 2021 Aug 9;11(8):604. doi: 10.3390/membranes11080604.

A primary biological function of multi-spanning membrane proteins is to transfer information and/or materials through a membrane by changing their conformations. Therefore, particular dynamics of the membrane proteins are tightly associated with their function. The semi-atomic resolution dynamics information revealed by NMR is able to discriminate function-related dynamics from random fluctuations. This review will discuss several studies in which quantitative dynamics information by solution NMR has contributed to revealing the structural basis of the function of multi-spanning membrane proteins, such as ion channels, GPCRs, and transporters.

多跨膜蛋白的一个主要生物学功能是通过改变其构象在膜上传递信息和/或物质。因此，膜蛋白的特定动力学与它们的功能紧密相关。核磁共振（NMR）揭示的半原子分辨率动力学信息能够区分与功能相关的动力学和随机波动。本综述将讨论几项研究，其中溶液核磁共振提供的定量动力学信息有助于揭示多跨膜蛋白（如离子通道、G蛋白偶联受体和转运蛋白）功能的结构基础。