采用液相色谱-串联质谱法比较分析比目鱼中 60 种兽药残留的样品前处理和测定。
Comparison of Sample Preparation and Determination of 60 Veterinary Drug Residues in Flatfish Using Liquid Chromatography-Tandem Mass Spectrometry.
机构信息
Pesticide and Veterinary Drug Residues Division, National Institute of Food and Drug Safety Evaluation, Osong, Chungcheongbuk-do 28165, Korea.
出版信息
Molecules. 2020 Mar 7;25(5):1206. doi: 10.3390/molecules25051206.
This study was performed to optimize the analytical method for multi-residues of 60 compounds in flatfish samples. Three sample preparation methods were tested to identify the optimal recovery conditions for target analytes. As a result, 10 mL of water/acetonitrile (1:4, /) was used to extract analytes from fish samples. For purification, C and 10 mL of acetonitrile saturated hexane were used to treat the samples. After evaporation and reconstitution, the fish samples were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry. The proposed method was validated according to the CODEX guidelines (CAC/GL-71). Our results showed the recoveries of 73.2%-115% and coefficients of variation of 1.6%-22.1%. The limit of quantification was 0.0005-0.005 mg/kg in the fishery products. In analysis of real samples, no samples exceeded the limit of quantification. This analytical method can be used for multi-residue screening and confirmation of the residues of veterinary drugs in fishery products.
本研究旨在优化检测比目鱼样品中 60 种化合物多残留的分析方法。测试了三种样品制备方法，以确定目标分析物的最佳回收条件。结果表明，用 10 mL 水/乙腈（1:4，/）从鱼样中提取分析物。为了净化，用 10 mL 乙腈饱和正己烷处理样品。蒸发和复溶后，采用超高效液相色谱-串联质谱法分析鱼样。该方法按照食品法典委员会（CAC/GL-71）准则进行了验证。结果显示，73.2%-115%的回收率和 1.6%-22.1%的变异系数。在渔业产品中，定量限为 0.0005-0.005 mg/kg。在实际样品分析中，没有样品超过定量限。该分析方法可用于渔业产品中兽药残留的多残留筛选和确证。
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