Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, 41125 Modena, Italy.
Biogest-Siteia, University of Modena and Reggio Emilia, Modena, Viale Amendola 2, 42122 Reggio Emilia, Italy.
Int J Mol Sci. 2020 Mar 7;21(5):1847. doi: 10.3390/ijms21051847.
Non- Enterobacterales (NECE) can colonize the human gut and may present virulence determinants and phenotypes that represent severe heath concerns. Most information is available for virulent NECE strains, isolated from patients with an ongoing infection, while the commensal NECE population of healthy subjects is understudied. In this study, 32 NECE strains were isolated from the feces of 20 healthy adults. 16S rRNA gene sequencing and mass spectrometry attributed the isolates to , , , , , , , sp., and , , and . Multiplex PCR revealed that harbored virulence genes for adhesins (, , and ) and enterobactin () and, in one case, also for yersiniabactin (, , , and ). Virulence genes were less numerous in the other NECE species. Biofilm formation was spread across all the species, while curli and cellulose were mainly produced by and . Among the most common antibiotics, amoxicillin-clavulanic acid was the sole against which resistance was observed, only strains being susceptible. The NECE inhabiting the intestine of healthy subjects have traits that may pose a health threat, taking into account the possibility of horizontal gene transfer.
非肠杆菌科（NECE）可以定植于人体肠道，可能具有潜在的毒力决定因子和表型，这对健康构成严重威胁。目前大多数信息都针对来自持续性感染患者的毒力 NECE 菌株，而健康受试者中的共生 NECE 群体研究较少。在这项研究中，从 20 位健康成年人的粪便中分离出 32 株 NECE 菌株。16S rRNA 基因测序和质谱分析将这些分离株归因于 、 、 、 、 、 sp.、 、 、 、 、 和 。多重 PCR 显示 携带黏附素（ 、 和 ）和 enterobactin（ ）的毒力基因，在一个病例中还携带耶尔森菌素（ 、 、 、 和 ）的毒力基因。其他 NECE 物种的毒力基因较少。所有物种均能形成生物膜，而 curli 和纤维素主要由 和 产生。在常用抗生素中，仅观察到对阿莫西林-克拉维酸的耐药性，仅有 株菌株敏感。考虑到水平基因转移的可能性，定植于健康受试者肠道的 NECE 具有潜在的健康威胁特征。
