School of Life Science of Nanchang University, Nanchang 330031, China; Department of Medical Genetics and Cell Biology, Medical College of Nanchang University, Nanchang 330006, China.
Department of Endocrinology and Metabolism, First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
Food Res Int. 2020 Apr;130:108939. doi: 10.1016/j.foodres.2019.108939. Epub 2019 Dec 24.
Propolis has beneficial effects anti-inflammatory, anti-diabetes and anti-obesity in human or murine models, but its mechanism has not been fully elucidated. This study was to investigate the effects of ethanol extract of propolis (EEP) on the gut microbiota, and to analyze the associations between these alterations of gut microbiota and insulin resistance and obesity in high fat diet (HFD)-fed mice. Male C57BL/6J mice were fed with chow diet, high-fat diet, and high-fat diet supplemented with 1% EEP or 2%EEP. EEP supplementation reduced HFD-induced weight gain and liver fat accumulation, proinflammatory cytokines and insulin resistance, and improved glucose tolerance and lipid profile. Meanwhile, EEP supplementation in HFD-fed mice increased anti-obesity and anti-inflammatory bacteria such as genera Roseburia and Intestinimonas and species Parabacteroides goldsteinii and Parabacteroides distasonis, and reduced pro-inflammatory bacteria such as genera Faecalibaculum and Prevotella and species Bacteroides vulgatus. These dominant bacterial taxa altered by EEP were significantly associated with the metabolic parameters of insulin resistance and obesityin HFD-fed mice. The results in this study indicated that EEP reduced HFD-induced obesity and insulin resistant, which may be mediated by modulating the composition and function of gut microbiota.
蜂胶具有抗炎、抗糖尿病和抗肥胖的作用，在人类或鼠类模型中都有，但它的作用机制尚未完全阐明。本研究旨在探讨蜂胶乙醇提取物（EEP）对肠道微生物群的影响，并分析这些肠道微生物群变化与高脂肪饮食（HFD）喂养小鼠胰岛素抵抗和肥胖之间的关联。雄性 C57BL/6J 小鼠分别用普通饲料、高脂肪饲料和高脂肪饲料加 1%EEP 或 2%EEP 喂养。EEP 补充剂可减少 HFD 引起的体重增加和肝脏脂肪堆积、促炎细胞因子和胰岛素抵抗，并改善葡萄糖耐量和脂质谱。同时，EEP 补充剂可增加 HFD 喂养小鼠中具有抗肥胖和抗炎作用的细菌，如罗斯伯里氏菌属和肠杆菌属，以及具有物种 Parabacteroides goldsteinii 和 Parabacteroides distasonis 的细菌，减少具有促炎作用的细菌，如粪杆菌属和普雷沃氏菌属以及物种脆弱拟杆菌。EEP 改变的这些主要细菌类群与 HFD 喂养小鼠胰岛素抵抗和肥胖的代谢参数显著相关。本研究结果表明，EEP 可减少 HFD 诱导的肥胖和胰岛素抵抗，这可能是通过调节肠道微生物群的组成和功能来介导的。
