Department of Cardiology and Internal Diseases, Military Institute of Medicine, Warsaw, Poland.
Institute of Biochemistry and Biophysics, PAS, Warsaw, Poland.
Sci Rep. 2020 Mar 10;10(1):4424. doi: 10.1038/s41598-020-61322-6.
MicroRNAs mediate posttranscriptional gene regulation. The aim of the study was to find a microRNA predictor of successful atrial fibrillation (AF) ablation. A total of 109 patients undergoing first-time AF ablation were included. Nineteen patients were selected to undergo serum microRNA sequencing (study group). The sequencing data were used to select several microRNAs that correlated with 12-month recurrences after AF ablation. Those microRNAs were validated by digital droplet PCR in samples from remaining 90 patients. All patients underwent pulmonary vein isolation (RF ablation, contact force catheter, electroanatomical system). The endpoint of the study was the 12-month AF recurrence rate; the overall recurrence rate was 42.5%. In total, levels of 34 miRNAs were significantly different in sera from patients with AF recurrence compared to patients without AF recurrence. Six microRNAs (miR-183-5p, miR-182-5p, miR-32-5p, miR-107, miR-574-3p, and miR-144-3p) were validated in the whole group. Data from the validation group did not confirm the observations from the study group, as no significant differences were found between miRNAs serum levels in patients with and without recurrences 12 months after AF ablation.
微小 RNA 介导转录后基因调控。本研究旨在寻找预测心房颤动 (AF) 消融成功的微小 RNA。共纳入 109 例首次接受 AF 消融的患者。19 例患者入选行血清微小 RNA 测序（研究组）。测序数据用于选择与 AF 消融后 12 个月复发相关的几种微小 RNA。这些微小 RNA 在剩余 90 例患者的样本中通过数字液滴 PCR 进行验证。所有患者均行肺静脉隔离（RF 消融、接触力导管、电解剖系统）。研究的终点是 12 个月的 AF 复发率；总的复发率为 42.5%。共有 34 种微小 RNA 的水平在有 AF 复发的患者血清中与无 AF 复发的患者明显不同。6 种微小 RNA（miR-183-5p、miR-182-5p、miR-32-5p、miR-107、miR-574-3p 和 miR-144-3p）在整个组中得到验证。验证组的数据未证实研究组的观察结果，因为在 AF 消融后 12 个月有和无复发的患者之间，微小 RNA 血清水平没有发现显著差异。
