Department of Periodontology, Faculty of Dentistry, Akdeniz University, Antalya, Turkey.
Department of Periodontology, Faculty of Dentistry, Atatürk University, Erzurum, Turkey.
Acta Odontol Scand. 2020 Aug;78(6):425-432. doi: 10.1080/00016357.2020.1734656. Epub 2020 Mar 11.
Vitamin C is an important water-soluble vitamin with antioxidant and immune-modulatory actions. The aim of this study was to investigate the effects of locally applied vitamin C on alveolar bone resorption in rats with experimental periodontitis. Twenty-one male Sprague-Dawley rats divided into three groups with seven animals in each group: (1) control, (2) experimental periodontitis and 3) experimental periodontitis-local vitamin C treatment group. After ligature was removed, 50 μL vitamin C was locally administered into the subperiosteum of the buccal gingiva of periodontitis vitamin C (PvitC) group rats for three times in intervals of 2 days. At the end of the study, the animals were scarified, and serum and gingival samples were collected for analysis of serum IL-1β, oxidative stress index (OSI), CTX and malondialdehyde (MDA) levels and gingival MMP-8 immunostaining. Alveolar bone loss and attachment loss were determined based on measurements on histological sections obtained from rat mandibles. Serum MDA and OSI levels which are related to the oxidative stress were significantly lower in the PvitC group as compared with those in the P group ( < .05). Serum CTX levels which are related to the bone resorption were significantly lower in the PvitC group as compared with those in the P group ( < .05). The numeric density of MMP-8-positive cells was significantly lower in the PvitC group compared to P group ( < .05). Alveolar bone loss and attachment loss were significantly lower in the PvitC group compared to P group ( < .05) The local vitamin C administration provided protection against inflammation-induced alveolar bone resorption by decreasing oxidative stress and inflammation-induced tissue breakdown vitamin C may be a therapeutic agent that can be used in periodontitis treatment.
维生素 C 是一种重要的水溶性维生素，具有抗氧化和免疫调节作用。本研究旨在探讨局部应用维生素 C 对实验性牙周炎大鼠牙槽骨吸收的影响。将 21 只雄性 Sprague-Dawley 大鼠分为三组，每组 7 只：（1）对照组；（2）牙周炎组；（3）牙周炎局部维生素 C 治疗组。结扎去除后，牙周炎维生素 C（PvitC）组大鼠的颊侧牙龈骨膜下局部给予 50μL 维生素 C，间隔 2 天给药 3 次。研究结束时，处死动物，采集血清和牙龈样本，用于分析血清 IL-1β、氧化应激指数（OSI）、CTX 和丙二醛（MDA）水平以及牙龈 MMP-8 免疫染色。根据大鼠下颌骨获得的组织学切片测量牙槽骨丢失和附着丧失。与 P 组相比，PvitC 组血清 MDA 和 OSI 水平（与氧化应激相关）显著降低（ < .05）。与 P 组相比，PvitC 组血清 CTX 水平（与骨吸收相关）显著降低（ < .05）。与 P 组相比，PvitC 组 MMP-8 阳性细胞的数值密度显著降低（ < .05）。与 P 组相比，PvitC 组牙槽骨丢失和附着丧失显著降低（ < .05）。局部维生素 C 给药通过降低氧化应激和炎症诱导的组织破坏，为炎症诱导的牙槽骨吸收提供了保护作用。维生素 C 可能是一种可用于牙周炎治疗的治疗剂。
