Price Paul T, Gaydos Thomas A, Berghaus Roy D, Baxter Virginia, Hofacre Charles L, Sims Michael D
Phileo by Lesaffre Milwaukee, Wisconsin, United States.
Department of Food, Nutrition, and Packaging Sciences Clemson University Clemson, South Carolina, United States.
Vet World. 2020 Jan;13(1):184-187. doi: 10.14202/vetworld.2020.184-187. Epub 2020 Jan 25.
Enteritidis (SE) is a significant foodborne pathogen that can often be traced to poultry and poultry products. This study aims to evaluate the ability of three commonly used non-antimicrobial feed additives in reducing the amount of SE in the ceca of laying type pullets.
On day 0, 60 Hy-Line Brown pullets aged 9 weeks were allocated to individual cages in 15 replicate blocks of four pens. Pullets were administered a mash feed provided without supplementation (control) or with dietary supplementation of 454 g/ton yeast cell wall (YCW), or 454 g/ton spp. probiotic, or 1133 g/ton yeast culture (YC). On day 3 of the trial, all birds were orally administered 3×10 CFU of a nalidixic acid-resistant SE. On day 10, 7 days after inoculation, all birds were humanely euthanized, and the ceca were aseptically removed for analysis.
There was no significant difference in the prevalence of SE among treatments. The mean quantity of SE detected in the ceca expressed in log most probable number/g was 2.52 in the control, 2.49 in the YCW treatment, 1.73 in the probiotic treatment, and 1.66 in the YC treatment. The reduction between control and probiotic and control and YC was significant (p=0.021).
This study demonstrates the ability of the novel probiotic and the YC to reduce the load of SE in layer ceca.
肠炎沙门氏菌（SE）是一种重要的食源性病原体，其源头通常可追溯至家禽及家禽产品。本研究旨在评估三种常用的非抗菌饲料添加剂降低蛋用型小母鸡盲肠中SE数量的能力。
在第0天，将60只9周龄的海兰褐小母鸡分配到15个重复组的单个笼子中，每个重复组有4个鸡笼。给小母鸡喂食不加补充剂的粉料（对照组），或添加454克/吨酵母细胞壁（YCW）、或454克/吨益生菌、或1133克/吨酵母培养物（YC）的日粮。在试验的第3天，所有鸡只口服接种3×10⁸CFU耐萘啶酸的SE。在第10天，即接种后7天，对所有鸡只实施安乐死，并无菌取出盲肠进行分析。
各处理组之间SE的流行率无显著差异。以每克对数最可能数表示，在盲肠中检测到的SE平均数量在对照组为2.52，在YCW处理组为2.49，在益生菌处理组为1.73，在YC处理组为1.66。对照组与益生菌组以及对照组与YC组之间的减少量具有显著差异（p=0.021）。
本研究证明了新型益生菌和YC能够降低蛋鸡盲肠中SE的载量。
