INSERM U1234, Normandy University, Rouen, France.
Department of Dermatology, Rouen University Hospital, Rouen, France.
Front Immunol. 2020 Feb 25;11:32. doi: 10.3389/fimmu.2020.00032. eCollection 2020.
CD11c B cells have been reported to be increased in autoimmune diseases, but they are detected in the blood of healthy individuals as well. We aimed to characterize CD11c B cells from healthy donors by flow cytometry, microarray analysis, and functional assays. Here, we report that CD11c B cells are a distinct subpopulation of B cells, enriched in the memory subpopulation even if their phenotype is heterogeneous, with overexpression of genes involved in B-cell activation and differentiation as well as in antigen presentation. Upon activation, CD11c B cells can differentiate into antibody-secreting cells, and CD11c could be upregulated in CD11c B cells by B-cell receptor activation. Finally, we show that patients with pemphigus, an autoimmune disease mediated by B cells, have a decreased frequency of CD11c B cell after treatment, relative to baseline. Our findings show that CD11c B cells are mainly memory B cells prone to differentiate into antibody secreting cells that accumulate with age, independently of gender.
CD11c B 细胞在自身免疫性疾病中被报道增加，但它们也存在于健康个体的血液中。我们旨在通过流式细胞术、微阵列分析和功能测定来对健康供体的 CD11c B 细胞进行特征分析。在这里，我们报告 CD11c B 细胞是 B 细胞的一个独特亚群，即使其表型是异质的，在记忆亚群中也得到了富集，其特征是与 B 细胞激活和分化以及抗原呈递相关的基因表达上调。在激活后，CD11c B 细胞可以分化为分泌抗体的细胞，并且 B 细胞受体的激活可以使 CD11c B 细胞中 CD11c 上调。最后，我们发现，在治疗后，与基线相比，天疱疮（一种由 B 细胞介导的自身免疫性疾病）患者的 CD11c B 细胞频率降低。我们的研究结果表明，CD11c B 细胞主要是记忆 B 细胞，容易分化为分泌抗体的细胞，并且这些细胞会随着年龄的增长而积累，与性别无关。
