Thangavelu Kiruthika, Sundararaju Pugalendhi, Srinivasan Naganandhini, Muniraj Iniyakumar, Uthandi Sivakumar
Department of Renewable Energy Engineering, Agricultural Engineering College and Research Institute, Coimbatore, Tamil Nadu 641 003 India.
2Biocatalysts Lab, Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu 641 003 India.
Biotechnol Biofuels. 2020 Mar 5;13:35. doi: 10.1186/s13068-020-01676-1. eCollection 2020.
Without sufficient alternatives to crude oil, as demand continues to rise, the global economy will undergo a drastic decline as oil prices explode. Dependence on crude oil and growing environmental impairment must eventually be overcome by creating a sustainable and profitable alternative based on renewable and accessible feedstock. One of the promising solutions for the current and near-future is the substitution of fossil fuels with sustainable liquid feedstock for biofuel production. Among the different renewable liquid feedstock's studied, wastewater is the least explored one for biodiesel production. Sago wastewater is the byproduct of the cassava processing industry and has starch content ranging from 4 to 7%. The present investigation was aimed to produce microbial lipids from oleaginous yeast, ASY2 for use as biodiesel feedstock and simultaneously decontaminate the sago processing wastewater for reuse. Initial screening of oleaginous yeast to find an efficient amylolytic with maximum lipid productivity resulted in a potent oleaginous yeast strain, ASY2, that utilizes SWW as a substrate. Shake flask experiments are conducted over a fermentation time of 240 h to determine a suitable fatty acid composition using GC-FID for biodiesel production with simultaneous removal of SWW pollutants using ASY2.
The maximum biomass of 0.021 g L h and lipid productivity of 0.010 g L h was recorded in SWW with lipid content of 49%. The yeast strain degraded cyanide in SWW (79%) and also removed chemical oxygen demand (COD), biological oxygen demand (BOD), nitrate (NO), ammoniacal (NH), and phosphate (PO) ions (84%, 92%, 100%, 98%, and 85%, respectively). GC-FID analysis of fatty acid methyl esters (FAME) revealed high oleic acid content (41.33%), which is one of the primary fatty acids for biodiesel production.
It is evident that the present study provides an innovative and ecologically sustainable technology that generates valuable fuel, biodiesel using SWW as a substrate and decontaminates for reuse.
由于缺乏足够的原油替代品，随着需求持续上升，一旦油价飙升，全球经济将急剧衰退。对原油的依赖以及日益严重的环境损害最终必须通过基于可再生且易于获取的原料创造一种可持续且有利可图的替代品来克服。对于当前及不久的将来，一个有前景的解决方案是用可持续液体原料替代化石燃料用于生物燃料生产。在研究的不同可再生液体原料中，废水是用于生物柴油生产研究最少的一种。西米废水是木薯加工业的副产品，淀粉含量在4%至7%之间。本研究旨在利用产油酵母ASY2生产微生物脂质用作生物柴油原料，同时净化西米加工废水以便再利用。对产油酵母进行初步筛选以找到具有最大脂质生产率的高效淀粉分解菌，结果得到了一种有效的产油酵母菌株ASY2，它利用西米废水作为底物。进行了240小时发酵时间内的摇瓶实验，使用气相色谱 - 火焰离子化检测器（GC - FID）确定适合生物柴油生产的脂肪酸组成，同时使用ASY2去除西米废水污染物。
在脂质含量为49%的西米废水中，记录到最大生物量为0.021 g/L·h，脂质生产率为0.010 g/L·h。该酵母菌株降解了西米废水中的氰化物（79%），还去除了化学需氧量（COD）、生物需氧量（BOD）、硝酸盐（NO）、氨（NH）和磷酸盐（PO）离子（分别为84%、92%、100%、98%和85%）。脂肪酸甲酯（FAME）的GC - FID分析显示油酸含量高（41.33%），这是生物柴油生产的主要脂肪酸之一。
显然，本研究提供了一种创新且生态可持续的技术，该技术以西米废水为底物生产有价值的燃料生物柴油，并进行净化以便再利用。
