Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.
Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
J Biomed Mater Res B Appl Biomater. 2020 Aug;108(6):2670-2680. doi: 10.1002/jbm.b.34598. Epub 2020 Mar 11.
Bone grafting procedures are commonly used to manage bone defects in the craniofacial region. Monetite is an excellent biomaterial option for bone grafting, however, it is limited by lack of osteoinduction. Several molecules can be incorporated within the monetite matrix to promote bone regeneration. The aim was to investigate whether incorporating bone forming drug conjugates (C3 and C6) within monetite can improve their ability to regenerate bone in bone defects. Bilateral bone defects were created in the mandible of 24 Sprague-Dawley rats and were then packed with monetite control, monetite+C3 or monetite+C6. After 2 and 4 weeks, post-mortem samples were analyzed using microcomputed tomography, histology and back-scattered electron microscopy to calculate the percentages of bone formation and remaining graft material. At 2 and 4 weeks, monetite with C3 and C6 demonstrated higher bone formation than monetite control, while monetite+C6 had the highest bone formation percentage at 4 weeks. There were no significant differences in the remaining graft material between the groups at 2 or 4 weeks. Incorporating these anabolic drug conjugates within the degradable matrix of monetite present a promising bone graft alternative for bone regeneration and repair in orthopedic as well as oral and maxillofacial applications.
骨移植手术常用于治疗颅面区域的骨缺损。磷灰石是一种优秀的骨移植生物材料选择，但其成骨诱导能力有限。几种分子可以被整合到磷灰石基质中，以促进骨再生。本研究旨在探讨在磷灰石中加入成骨药物偶联物（C3 和 C6）是否可以提高其在骨缺损中再生骨的能力。在 24 只 Sprague-Dawley 大鼠的下颌骨中创建双侧骨缺损，然后用磷灰石对照、磷灰石+C3 或磷灰石+C6 填充。在 2 周和 4 周后，通过体视学、组织学和背散射电子显微镜对死后样本进行分析，以计算骨形成和剩余移植物材料的百分比。在 2 周和 4 周时，与磷灰石对照相比，含 C3 和 C6 的磷灰石具有更高的骨形成率，而在 4 周时，含 C6 的磷灰石具有最高的骨形成率。在 2 周和 4 周时，各组之间剩余移植物材料无显著差异。将这些合成代谢药物偶联物整合到可降解的磷灰石基质中，为骨科以及口腔颌面应用中的骨再生和修复提供了一种有前途的骨移植替代物。
