Laboratorio de Química de Macroalgas, Centro Interdisciplinario de Ciencias Marinas, Instituto Politécnico Nacional. Av. Instituto Politécnico, La Paz, B.C.S. México.
Laboratorio de Diseño y Desarrollo de Nuevos Fármacos e Innovación Biotecnológica (Laboratory for the Design and Development of New Drugs and Biotechnological Innovation), Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México.
J Biomol Struct Dyn. 2021 Mar;39(4):1271-1283. doi: 10.1080/07391102.2020.1729863. Epub 2020 Mar 11.
The number of patients diagnosed with Alzheimer's disease (AD) increases each year, and there are currently few treatment strategies to decrease the symptoms of AD; furthermore, these strategies are not sufficient to reduce memory loss in AD patients. In this work and studies were performed to evaluate the effects of fucosterol, which was extracted from an algal source and characterized by liquid chromatography-mass spectra (LC-MS), as an inhibitor of Aβ aggregation. Experimental studies, including protein gel electrophoresis, atomic force microscopy and fluorescence studies with thioflavin T (ThT), highlighted that fucosterol can decrease oligomer formation more than galantamine, which was used as a positive control. Docking and molecular dynamics simulations coupled with an MMGBSA approach showed that fucosterol is capable of recognizing the hydrophobic regions of monomeric Aβ, suggesting that fucosterol could affect amyloid-beta (Aβ) aggregation by preventing the formation of oligomers, preventing the development of AD.Communicated by Ramaswamy H. Sarma.
每年被诊断患有阿尔茨海默病（AD）的患者人数都在增加，目前针对 AD 症状的治疗策略很少；此外，这些策略还不足以减少 AD 患者的记忆丧失。在这项工作中，[作者姓名]进行了研究，以评估从藻类中提取的岩藻甾醇作为 Aβ 聚集抑制剂的效果。通过液相色谱-质谱（LC-MS）进行了包括蛋白质凝胶电泳、原子力显微镜和硫黄素 T（ThT）荧光研究在内的实验研究，结果表明岩藻甾醇可以比加兰他敏（用作阳性对照）更有效地减少低聚物的形成。对接和分子动力学模拟与 MMGBSA 方法相结合的结果表明，岩藻甾醇能够识别单体 Aβ 的疏水区，这表明岩藻甾醇可以通过阻止寡聚物的形成、防止 AD 的发展来影响β淀粉样蛋白（Aβ）的聚集。由 Ramaswamy H. Sarma 交流。
